Literature DB >> 165929

Fluorimetric determination of dipeptidyl carboxypeptidase. (angiotensin-I-converting enzyme).

D Depierre, M Roth.   

Abstract

A fluorimetric assay of dipeptidyl carboxypeptidase is described. It involves incubation at 37 degrees C with the substrate, Z-Phe-His-Leu, and reaction of the dipeptide His-Leu which is released upon enzymatic hydrolysis, with o-phthalaldehyde to yield a fluorescent compound. The method is simple, precise and sensitive. The assay in serum is conveniently performed on 20 mul samples. Normal values in human serum range from 0.04 to 0.22 U/l.

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Year:  1975        PMID： 165929     DOI： 10.1159/000458975

Source DB:  PubMed          Journal:  Enzyme        ISSN： 0013-9432


  8 in total

1.  Angiotensin I converting enzyme in cerebrospinal fluid of patients with neurological diseases.

Authors:  H Schweisfurth; S Schiöberg-Schiegnitz; W Kuhn; B Parusel
Journal:  Klin Wochenschr       Date:  1987-10-15

2.  Angiotensin-converting enzyme in sarcoidosis and other pulmonary diseases: a comparison of two methods of determination.

Authors:  D Sandron; D Lecossier; F Moreau; A Grodet; G Basset; J P Battesti
Journal:  Lung       Date:  1979       Impact factor: 2.584

3.  No correlation between circulating ACE activity and VO2max or mechanical efficiency in women.

Authors:  Stephen H Day; Peter Gohlke; Sukhbir S Dhamrait; Alun G Williams
Journal:  Eur J Appl Physiol       Date:  2006-09-28       Impact factor: 3.078

4.  Comparative study on the selenium- and N-acetylcysteine-related effects on the toxic action of hyperoxia, paraquat and the enzyme reaction hypoxanthine-xanthine oxidase in cultured endothelial cells.

Authors:  A F Junod; L Jornot; G Grichting
Journal:  Agents Actions       Date:  1987-10

5.  Cardioprotective actions of wild garlic (allium ursinum) in ischemia and reperfusion.

Authors:  B Rietz; H Isensee; H Strobach; S Makdessi; R Jacob
Journal:  Mol Cell Biochem       Date:  1993-02-17       Impact factor: 3.396

6.  Renin, angiotensins, and angiotensin-converting enzyme in neuroblastoma cells: evidence for intracellular formation of angiotensins.

Authors:  T Okamura; D L Clemens; T Inagami
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

7.  The acute effects of exercise and glucose ingestion on circulating angiotensin-converting enzyme in humans.

Authors:  S H Day; C Williams; J P Folland; P Gohlke; A G Williams
Journal:  Eur J Appl Physiol       Date:  2004-08       Impact factor: 3.078

8.  Mitochondrial uncoupling proteins regulate angiotensin-converting enzyme expression: crosstalk between cellular and endocrine metabolic regulators suggested by RNA interference and genetic studies.

Authors:  Sukhbir S Dhamrait; Cecilia Maubaret; Ulrik Pedersen-Bjergaard; David J Brull; Peter Gohlke; John R Payne; Michael World; Birger Thorsteinsson; Steve E Humphries; Hugh E Montgomery
Journal:  Inside Cell       Date:  2015-08-02
  8 in total

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