Literature DB >> 16592679

Iron-dextran antibody conjugates: General method for simultaneous staining of two components in high-resolution immunoelectron microscopy.

A H Dutton1, K T Tokuyasu, S J Singer.   

Abstract

We report the preparation and properties of an electron-dense antibody conjugate, made by the covalent bonding of an iron-dextran (Imposil) particle to an antibody molecule. Transmission electron microscopic experiments with the Imposil-antibody conjugates demonstrate their suitability as specific immunostains at high resolution, particularly for simultaneous double staining experiments in conjunction with ferritin-antibody conjugates.

Entities:  

Year:  1979        PMID： 16592679      PMCID： PMC383831          DOI： 10.1073/pnas.76.7.3392

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  19 in total

1.  Preparation of an electron-dense antibody conjugate.

Authors:  S J SINGER
Journal:  Nature       Date:  1959-05-30       Impact factor: 49.962

2.  Detection and ultrastructural localization of human smooth muscle myosin-like molecules in human non-muscle cells by specific antibodies.

Authors:  R G Painter; M Sheetz; S J Singer
Journal:  Proc Natl Acad Sci U S A       Date:  1975-04       Impact factor: 11.205

3.  Enzyme-labeled antibodies: preparation and application for the localization of antigens.

Authors:  P K Nakane; G B Pierce
Journal:  J Histochem Cytochem       Date:  1966-12       Impact factor: 2.479

4.  Colloidal gold, a useful marker for transmission and scanning electron microscopy.

Authors:  M Horisberger; J Rosset
Journal:  J Histochem Cytochem       Date:  1977-04       Impact factor: 2.479

5.  Rapid induction of ferritin in laboratory animals prior to its isolation.

Authors:  E Avol; D Carmichael; J Hegenauer; P Saltman
Journal:  Prep Biochem       Date:  1973

6.  New visual markers of antibody for electron microscopy.

Authors:  U Hämmerling; T Aoki; H A Wood; L J Old; E A Boyse; E de Harvin
Journal:  Nature       Date:  1969-09-13       Impact factor: 49.962

7.  A study of positive staining of ultrathin frozen sections.

Authors:  K T Tokuyasu
Journal:  J Ultrastruct Res       Date:  1978-06

8.  The iron-dextran complex.

Authors:  C R Ricketts; J S Cox; C Fitzmaurice; G F Moss
Journal:  Nature       Date:  1965-10-16       Impact factor: 49.962

9.  Improved procedures for immunoferritin labeling of ultrathin frozen sections.

Authors:  K T Tokuyasu; S J Singer
Journal:  J Cell Biol       Date:  1976-12       Impact factor: 10.539

10.  New immunolatex spheres: visual markers of antigens on lymphocytes for scanning electron microscopy.

Authors:  R S Molday; W J Dreyer; A Rembaum; S P Yen
Journal:  J Cell Biol       Date:  1975-01       Impact factor: 10.539
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  19 in total

1.  Immunogold cytochemistry in neuroscience.

Authors:  Mahmood Amiry-Moghaddam; Ole Petter Ottersen
Journal:  Nat Neurosci       Date:  2013-06-25       Impact factor: 24.884

2.  Mutants of Actinomyces viscosus T14V lacking type 1, type 2, or both types of fimbriae.

Authors:  J O Cisar; A E Vatter; W B Clark; S H Curl; S Hurst-Calderone; A L Sandberg
Journal:  Infect Immun       Date:  1988-11       Impact factor: 3.441

3.  Multiple-labelling immunoEM using different sizes of colloidal gold: alternative approaches to test for differential distribution and colocalization in subcellular structures.

Authors:  Terry M Mayhew; John M Lucocq
Journal:  Histochem Cell Biol       Date:  2011-02-16       Impact factor: 4.304

4.  Passage of an integral membrane protein, the vesicular stomatitis virus glycoprotein, through the Golgi apparatus en route to the plasma membrane.

Authors:  J E Bergmann; K T Tokuyasu; S J Singer
Journal:  Proc Natl Acad Sci U S A       Date:  1981-03       Impact factor: 11.205

5.  Altered distributions of the cytoskeletal proteins vinculin and alpha-actinin in cultured fibroblasts transformed by Rous sarcoma virus.

Authors:  T David-Pfeuty; S J Singer
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

6.  An improved procedure for immunoelectron microscopy: ultrathin plastic embedding of immunolabeled ultrathin frozen sections.

Authors:  G A Keller; K T Tokuyasu; A H Dutton; S J Singer
Journal:  Proc Natl Acad Sci U S A       Date:  1984-09       Impact factor: 11.205

7.  Visualization of antigens attached to cytoskeletal framework in animal cells: colocalization of simian virus 40 Vp1 polypeptide and actin in TC7 cells.

Authors:  H Kasamatsu; W Lin; J Edens; J P Revel
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

8.  Immunochemistry on ultrathin frozen sections.

Authors:  K T Tokuyasu
Journal:  Histochem J       Date:  1980-07

9.  Saposin C coupled lipid nanovesicles enable cancer-selective optical and magnetic resonance imaging.

Authors:  Vinod Kaimal; Zhengtao Chu; Yonatan Y Mahller; Brigitte Papahadjopoulos-Sternberg; Timothy P Cripe; Scott K Holland; Xiaoyang Qi
Journal:  Mol Imaging Biol       Date:  2011-10       Impact factor: 3.488

10.  Whole-cell-mount cytochemistry by the colloidal gold labeling method. Combined transmission and scanning electron microscopic study of ConA binding sites in mouse macrophages.

Authors:  K Takata; H Hirano
Journal:  Histochemistry       Date:  1984
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