OBJECTIVE: Pro-inflammatory cytokines, such as tumor necrosis factor alpha (TNF-alpha), stimulate the release of C-reactive protein (CRP). We investigated the association between the TNF-alpha G-308A polymorphism and plasma CRP levels. METHODS: Subjects were 456 White (225 men, 231 women) and 232 Black (83 men, 149 women) healthy adults who underwent a 20-week standardized exercise program in the HERITAGE Family Study. The TNF-alpha gene promoter polymorphism was determined using PCR amplification followed by NcoI digestion. Plasma CRP was measured using a high-sensitivity assay. RESULTS: Genotype frequencies were in Hardy-Weinberg equilibrium. After adjustment for age, smoking, alcohol consumption, maximal oxygen uptake and, in women, hormone use, the AA homozygotes for the G-308A polymorphism had higher baseline CRP levels than other genotypes in White and Black men (P<0.001 and P = 0.044, respectively) and in Black women (P = 0.032). Body mass index partly explained these associations in Blacks. The exercise program results provided further evidence for an association with the polymorphism. Among those with high CRP at baseline (> or = 3.0 mg/L), regular exercise decreased CRP less in AA homozygotes than in other genotypes (P = 0.043). CONCLUSION: The AA genotype of the TNF-alpha G-308A polymorphism is associated with higher plasma CRP levels and less favorable CRP response to regular exercise.
OBJECTIVE: Pro-inflammatory cytokines, such as tumor necrosis factor alpha (TNF-alpha), stimulate the release of C-reactive protein (CRP). We investigated the association between the TNF-alphaG-308A polymorphism and plasma CRP levels. METHODS: Subjects were 456 White (225 men, 231 women) and 232 Black (83 men, 149 women) healthy adults who underwent a 20-week standardized exercise program in the HERITAGE Family Study. The TNF-alpha gene promoter polymorphism was determined using PCR amplification followed by NcoI digestion. Plasma CRP was measured using a high-sensitivity assay. RESULTS: Genotype frequencies were in Hardy-Weinberg equilibrium. After adjustment for age, smoking, alcohol consumption, maximal oxygen uptake and, in women, hormone use, the AA homozygotes for the G-308A polymorphism had higher baseline CRP levels than other genotypes in White and Black men (P<0.001 and P = 0.044, respectively) and in Black women (P = 0.032). Body mass index partly explained these associations in Blacks. The exercise program results provided further evidence for an association with the polymorphism. Among those with high CRP at baseline (> or = 3.0 mg/L), regular exercise decreased CRP less in AA homozygotes than in other genotypes (P = 0.043). CONCLUSION: The AA genotype of the TNF-alphaG-308A polymorphism is associated with higher plasma CRP levels and less favorable CRP response to regular exercise.
Authors: Mark A Sarzynski; Treva K Rice; Jean-Pierre Després; Louis Pérusse; Angelo Tremblay; Philip R Stanforth; André Tchernof; Jacob L Barber; Francesco Falciani; Clary Clish; Jeremy M Robbins; Sujoy Ghosh; Robert E Gerszten; Arthur S Leon; James S Skinner; D C Rao; Claude Bouchard Journal: Med Sci Sports Exerc Date: 2022-05-01
Authors: Matthew T Haren; Theodore K Malmstrom; Douglas K Miller; Ping Patrick; H M Perry; Margaret M Herning; William A Banks; John E Morley Journal: J Gerontol A Biol Sci Med Sci Date: 2009-10-07 Impact factor: 6.053