BACKGROUND: Autologous recellularization of decellularized heart valve scaffolds is a promising challenge in the field of tissue-engineered heart valves and could be boosted by bone marrow progenitor cell mobilization. The aim of this study was to examine the spontaneous in vivo recolonization potential of xenogeneic decellularized heart valves in a lamb model and the effects of granulocyte colony-stimulating factor mobilization of bone marrow cells on this process. METHODS: Decellularized porcine aortic valves were implanted in 12 lambs. Six lambs received granulocyte colony-stimulating factor (10 microg x kg(-1) x d(-1) for 7 days, granulocyte colony-stimulating factor group), and 6 received no granulocyte colony-stimulating factor (control group). Additionally, nondecellularized porcine valves were implanted in 5 lambs (xenograft group). Angiographic and histologic evaluation was performed at 3, 6, 8, and 16 weeks. RESULTS: Few macroscopic modifications of leaflets and the aortic wall were observed in the control group, whereas progressive shrinkage and thickening of the leaflets appeared in the granulocyte colony-stimulating factor and xenograft groups. In the 3 groups progressive ovine cell infiltration (fluorescence in situ hybridization) was observed in the leaflets and in the adventitia and the intima of the aortic wall but not in the media. Neointimal proliferation of alpha-actin-positive cells, inflammatory infiltration, adventitial neovascularization, and calcifications were more important in the xenograft and the granulocyte colony-stimulating factor groups than in the control group. Continuous re-endothelialization appeared only in the control group. CONCLUSION: Decellularized xenogeneic heart valve scaffolds allowed partial autologous recellularization. Granulocyte colony-stimulating factor led to accelerated heart valve deterioration similar to that observed in nondecellularized xenogeneic cardiac bioprostheses.
BACKGROUND: Autologous recellularization of decellularized heart valve scaffolds is a promising challenge in the field of tissue-engineered heart valves and could be boosted by bone marrow progenitor cell mobilization. The aim of this study was to examine the spontaneous in vivo recolonization potential of xenogeneic decellularized heart valves in a lamb model and the effects of granulocyte colony-stimulating factor mobilization of bone marrow cells on this process. METHODS: Decellularized porcine aortic valves were implanted in 12 lambs. Six lambs received granulocyte colony-stimulating factor (10 microg x kg(-1) x d(-1) for 7 days, granulocyte colony-stimulating factor group), and 6 received no granulocyte colony-stimulating factor (control group). Additionally, nondecellularized porcine valves were implanted in 5 lambs (xenograft group). Angiographic and histologic evaluation was performed at 3, 6, 8, and 16 weeks. RESULTS: Few macroscopic modifications of leaflets and the aortic wall were observed in the control group, whereas progressive shrinkage and thickening of the leaflets appeared in the granulocyte colony-stimulating factor and xenograft groups. In the 3 groups progressive ovine cell infiltration (fluorescence in situ hybridization) was observed in the leaflets and in the adventitia and the intima of the aortic wall but not in the media. Neointimal proliferation of alpha-actin-positive cells, inflammatory infiltration, adventitial neovascularization, and calcifications were more important in the xenograft and the granulocyte colony-stimulating factor groups than in the control group. Continuous re-endothelialization appeared only in the control group. CONCLUSION: Decellularized xenogeneic heart valve scaffolds allowed partial autologous recellularization. Granulocyte colony-stimulating factor led to accelerated heart valve deterioration similar to that observed in nondecellularized xenogeneic cardiac bioprostheses.
Authors: Adriana Harbuzariu; Justine Kim; E Michael Meyer; Albert D Donnenberg; Bryan W Tillman Journal: J Vasc Surg Date: 2013-07-19 Impact factor: 4.268
Authors: Ryan S Hennessy; Soumen Jana; Brandon J Tefft; Meghana R Helder; Melissa D Young; Rebecca R Hennessy; Nicholas J Stoyles; Amir Lerman Journal: JACC Basic Transl Sci Date: 2017-02
Authors: Paola Lanuti; Francesco Serafini; Laura Pierdomenico; Pasquale Simeone; Giuseppina Bologna; Eva Ercolino; Sara Di Silvestre; Simone Guarnieri; Carlo Canosa; Gianna Gabriella Impicciatore; Stella Chiarini; Francesco Magnacca; Maria Addolorata Mariggiò; Assunta Pandolfi; Marco Marchisio; Gabriele Di Giammarco; Sebastiano Miscia Journal: Biores Open Access Date: 2015-06-01
Authors: Carlos Landa-Solís; Julio Granados-Montiel; Anell Olivos-Meza; Carmina Ortega-Sánchez; Mónica Cruz-Lemini; Cecilia Hernández-Flores; María Eugenia Chang-González; Ricardo Gómez García; Brenda Olivos-Díaz; María Cristina Velasquillo-Martínez; Carlos Pineda; Clemente Ibarra Journal: Cell Tissue Bank Date: 2015-07-29 Impact factor: 1.522