[Functional analyses of TcrA-a TPR-containing regulatory protein in Streptomyces coelicolor A3(2)].

In Streptomyces coelicolor A3(2), SCO5433 encodes a TPR domain containing protein designated TcrA (TPR containing regulator A). TcrA is similar in amino acid sequence to AfsR, a well-characterized global regulatory protein in S. coelicolor A3(2). Disruption of tcrA enhanced the production of spore pigment on MM containing glucose or mannitol and also resulted in more diffusible pigment production on MM containing mannitol or MS agar medium, but no significant effects on morphological differentiation were observed. Complementation of tcrA mutation restored the phenotype of tcrA mutant to that of the wild-type strain. These results suggest that tcrA is involved in the regulation of secondary metabolism under defined conditions. In S. coelicolor A3(2), the AfsK/AfsR system positively regulates the production of secondary metabolites while the results of our work suggest that there might be a TcrA-dependent pathway that negatively regulates secondary metabolism.