Literature DB >> 16578765

Procedure for rapid isolation of photosynthetic reaction centers using cytochrome c affinity chromatography.

G W Brudvig1, S T Worland, K Sauer.   

Abstract

Horse heart cytochrome c linked to Sepharose 4B is used to purify reaction centers from Rhodopseudomonas sphaeroides R-26. This procedure allows for an initial recovery of 80-90% of the bacterial reaction centers present in chromatophore membranes. High purity reaction centers (A(280)/A(802) < 1.30) can be obtained with a 30% recovery. Reaction centers from wild-type Rps. sphaeroides and Rps. capsulata also bind to a cytochrome c column. Cytochrome c affinity chromatography can also be used to isolate photosystem I complexes from spinach chloroplasts.

Entities:  

Year:  1983        PMID： 16578765      PMCID： PMC393443          DOI： 10.1073/pnas.80.3.683

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  11 in total

1.  Kinetic studies of pigment synthesis by non-sulfur purple bacteria.

Authors:  G COHEN-BAZIRE; W R SISTROM; R Y STANIER
Journal:  J Cell Comp Physiol       Date:  1957-02

2.  Separation of right-side-out and inside-out submitochondrial particles by affinity chromatography on Sepharose-cytochrome c.

Authors:  C Godinot; D C Gautheron
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

Review 3.  Membrane-bound iron-sulfur centers in photosynthetic systems.

Authors:  R Malkin; A J Bearden
Journal:  Biochim Biophys Acta       Date:  1978-10-23

4.  COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

Authors:  D I Arnon
Journal:  Plant Physiol       Date:  1949-01       Impact factor: 8.340

5.  Use of immobilized light-harvesting chlorophyll a/b protein to study the stoichiometry of its self-association.

Authors:  M Takahashi; E L Gross
Journal:  Biochemistry       Date:  1978-03-07       Impact factor: 3.162

6.  Characterization of reaction centers from photosynthetic bacteria. I. Subunit structure of the protein mediating the primary photochemistry in Rhodopseudomonas spheroides R-26.

Authors:  M Y Okamura; L A Steiner; G Feher
Journal:  Biochemistry       Date:  1974-03-26       Impact factor: 3.162

7.  A rapid procedure for the isolation and purification of photosynthetic reaction centers from Rhodopseudomonas sphaeroides R-26.

Authors:  M W Kendall-Tobias; M Seibert
Journal:  Arch Biochem Biophys       Date:  1982-06       Impact factor: 4.013

8.  Further studies on the composition and spectral properties of the photochemical reaction centers of bacteriochlorophyll b-containing bacteria.

Authors:  J P Thornber; R J Cogdell; R E Seftor; G D Webster
Journal:  Biochim Biophys Acta       Date:  1980-11-05

9.  Interaction of cytochrome c with reaction centers of Rhodopseudomonas sphaeroides R-26: determination of number of binding sites and dissociation constants by equilibrium dialysis.

Authors:  D Rosen; M Y Okamura; G Feher
Journal:  Biochemistry       Date:  1980-12-09       Impact factor: 3.162

10.  Effect of detergents on the reliability of a chemical assay for P-700.

Authors:  J P Markwell; J P Thornber; M P Skrdla
Journal:  Biochim Biophys Acta       Date:  1980-07-08
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  3 in total

1.  Reaction centers from three herbicide-resistant mutants of Rhodobacter sphaeroides 2.4.1: sequence analysis and preliminary characterization.

Authors:  M L Paddock; S H Rongey; E C Abresch; G Feher; M Y Okamura
Journal:  Photosynth Res       Date:  1988-07       Impact factor: 3.573

Review 2.  The electronic structure of Fe2+ in reaction centers from Rhodopseudomonas sphaeroides. III. EPR measurements of the reduced acceptor complex.

Authors:  W F Butler; R Calvo; D R Fredkin; R A Isaacson; M Y Okamura; G Feher
Journal:  Biophys J       Date:  1984-05       Impact factor: 4.033

3.  Labeling quinone-binding sites in photosynthetic reaction centers: A 38-kilodalton protein associated with the acceptor side of photosystem II.

Authors:  S T Worland; A Yamagishi; S Isaacs; K Sauer; J E Hearst
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205
  3 in total

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