| Literature DB >> 1657407 |
X F Wang1, H Y Lin, E Ng-Eaton, J Downward, H F Lodish, R A Weinberg.
Abstract
The rat TGF-beta type III receptor cDNA has been cloned by overexpression in COS cells. The encoded receptor is an 853 amino acid protein with a large N-terminal extracellular domain containing at least one site for glycosaminoglycan addition, a single hydrophobic transmembrane domain, and a 41 amino acid cytoplasmic tail with no obvious signaling motif. Introduction of the cDNA into COS cells and L6 myoblasts induces expression of a heterogenously glycosylated 280-330 kd protein characteristic of the type III receptor that binds TGF-beta 1 specifically. In L6 myoblasts lacking the endogenous type III receptor, expression of the recombinant receptor leads to an increase in the amount of ligand bound and cross-linked to surface type II TGF-beta receptors. This indicates that the type III receptor may regulate the ligand-binding ability or surface expression of the type II receptor.Entities:
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Year: 1991 PMID： 1657407 DOI： 10.1016/0092-8674(91)90074-9
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582