OBJECTIVE: The purpose of the study was to determine the frequency of C.trachomatis and HPV infections in teenage, sexually active girls. MATERIAL AND METHODS: 48 non-pregnant, sexually active young women (between 16-19 years of age), who submitted for periodical check-up during oral contraceptive use. Patients had periodical mild pain in the lower abdominal area and increased amount of mucigenous secretion. They had their first sexual intercourse before 16 years of age, multiple partners (>3), unprotected intercourse. The direct immunofluorescence (DIF) was used for C. trachomatis antigen detection (Chlamydia Direct IF (bioMérieux, France). DNA HPV presence and oncogenic potential was assessed using hybridization method (Hybrid Capture I HPV Assay, Digene Corp. Beltsville, MD U.S.A.). RESULTS: C. trachomatis antigen was detected in 13 aut of 48 swabs (27%), presence of HPV DNA was detected in 2 teenage girls with normal cytology (4.2%). In cytological evaluation LSIL (low grade squamous intraepithelial lesions) was described in 5 cases. In 4 of them along with LSIL coexisted C. trachomatis infection.
OBJECTIVE: The purpose of the study was to determine the frequency of C.trachomatis and HPV infections in teenage, sexually active girls. MATERIAL AND METHODS: 48 non-pregnant, sexually active young women (between 16-19 years of age), who submitted for periodical check-up during oral contraceptive use. Patients had periodical mild pain in the lower abdominal area and increased amount of mucigenous secretion. They had their first sexual intercourse before 16 years of age, multiple partners (>3), unprotected intercourse. The direct immunofluorescence (DIF) was used for C. trachomatis antigen detection (Chlamydia Direct IF (bioMérieux, France). DNA HPV presence and oncogenic potential was assessed using hybridization method (Hybrid Capture I HPV Assay, Digene Corp. Beltsville, MD U.S.A.). RESULTS:C. trachomatis antigen was detected in 13 aut of 48 swabs (27%), presence of HPV DNA was detected in 2 teenage girls with normal cytology (4.2%). In cytological evaluation LSIL (low grade squamous intraepithelial lesions) was described in 5 cases. In 4 of them along with LSIL coexisted C. trachomatis infection.