Literature DB >> 16557708

Purification and Characterization of Two Proteins from Culture Filtrates of Mycobacterium tuberculosis H(37)Ra Strain.

T M Daniel1, L E Ferguson.   

Abstract

Two proteins have been purified from culture filtrates of Mycobacterium tuberculosis, H(37)Ra strain by a procedure combining gel filtration, diethylaminoethyl (DEAE)-cellulose chromatography, and zone electrophoresis. The two proteins are similar in molecular weight but differ slightly in charge. The faster migrating protein, designated a(1), is not antigenic. The slower migrating protein, designated a(2), is antigenic both with respect to antisera and as a skin-testing antigen.

Entities:  

Year:  1970        PMID： 16557708      PMCID： PMC415872          DOI： 10.1128/iai.1.2.164-168.1970

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  13 in total

1.  MYCOBACTERIAL ANTIGENS: A STUDY OF ANTIGENS IN BACTERIAL EXTRACTS AND IN CULTURE FILTRATES OF DIFFERENT AGES.

Authors:  G CASTELNUOVO; M E DUNCAN; G BELLEZZA
Journal:  Tubercle       Date:  1964-09

2.  ELECTROPHORESIS ON POLYACRYLAMIDE GELS OF PROTEIN AND POLYSACCHARIDE FRACTIONS FROM MYCOBACTERIUM TUBERCULOSIS.

Authors:  L F AFFRONTI; R C PARLETT; R A CORNESKY
Journal:  Am Rev Respir Dis       Date:  1965-01

3.  THE SEPARATION OF MYCOBACTERIAL ANTIGENS BY ION-EXCHANGE CHROMATOGRAPHY. I. DETAILS OF CHROMATOGRAPHIC AND IMMUNOLOGIC PROCEDURES AND RESULTS WITH FOUR STRAINS OF M. TUBERCULOSIS.

Authors:  W T KNIKER; J B LABORDE
Journal:  Am Rev Respir Dis       Date:  1964-01

4.  THE IMMUNOLOGY AND CHEMISTRY OF TUBERCULIN. II. CHROMATOGRAPHY WITH SEPHADEX OF THE NONDIALYZABLE TUBERCULIN-ACTIVE CONSTITUENTS OF BCG CULTURE FILTRATE.

Authors:  S D CHAPARAS; H BAER
Journal:  Am Rev Respir Dis       Date:  1964-01

5.  Extracellular proteins of tubercle bacilli. III. Column chromatographical purification of the proteins of a virulent strain (H37Rv) of Mycobacterium tuberculosis.

Authors:  Y FUKUI; M YONEDA
Journal:  Biken J       Date:  1961-09

6.  Serological studies of mycobacteria by means of diffusion-in-gel techniques. VI. Physical, chemical and immunological characteristics of precipitinogenic factors in an antigenic preparation from a culture filtrate of a virulent strain of M. tuberculosis.

Authors:  A LIND
Journal:  Int Arch Allergy Appl Immunol       Date:  1961

7.  A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Authors:  R G MARTIN; B N AMES
Journal:  J Biol Chem       Date:  1961-05       Impact factor: 5.157

8.  High-Resolution Density Gradient Sedimentation Analysis.

Authors:  R J Britten; R B Roberts
Journal:  Science       Date:  1960-01-01       Impact factor: 47.728

9.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

10.  A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis.

Authors:  A Chrambach; R A Reisfeld; M Wyckoff; J Zaccari
Journal:  Anal Biochem       Date:  1967-07       Impact factor: 3.365
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  22 in total

1.  Serological response of patients with leprosy to a 28- to 30-kilodalton protein doublet from early cultures of Mycobacterium bovis BCG.

Authors:  M C Pessolani; F D Rumjanek; M A Marques; F S de Melo; E N Sarno
Journal:  J Clin Microbiol       Date:  1989-10       Impact factor: 5.948

2.  The bentonite granuloma. Characterization of a model system for infectious and foreign body granulomatous inflammation using soluble mycobacterial, histoplasma and schistosoma antigens.

Authors:  D L Boros; K S Warren
Journal:  Immunology       Date:  1973-03       Impact factor: 7.397

3.  Demonstration of a shared epitope among mycobacterial antigens using a monoclonal antibody.

Authors:  T M Daniel; G R Olds
Journal:  Clin Exp Immunol       Date:  1985-05       Impact factor: 4.330

Review 4.  Defining the humoral immune response to infectious agents using high-density protein microarrays.

Authors:  Adam Vigil; D Huw Davies; Philip L Felgner
Journal:  Future Microbiol       Date:  2010-02       Impact factor: 3.165

Review 5.  Mycobacterial antigens: a review of their isolation, chemistry, and immunological properties.

Authors:  T M Daniel; B W Janicki
Journal:  Microbiol Rev       Date:  1978-03

6.  Specificity of Mycobacterium tuberculosis antigen 5 determined with mouse monoclonal antibodies.

Authors:  T M Daniel; N J Gonchoroff; J A Katzmann; G R Olds
Journal:  Infect Immun       Date:  1984-07       Impact factor: 3.441

7.  A longitudinal study of antibody titres to Antigen 6 in patients with bone and joint tuberculosis.

Authors:  J H Lau; J C Leong; A B Stroebel
Journal:  Int Orthop       Date:  1983       Impact factor: 3.075

8.  Specific skin-reactive protein from culture filtrate of Mycobacterium bovis BCG.

Authors:  S Nagai; J Matsumoto; T Nagasuga
Journal:  Infect Immun       Date:  1981-03       Impact factor: 3.441

9.  Heterogeneity of monoclonal antibody-reactive epitopes on mycobacterial 30-kilodalton-region proteins and the secreted antigen 85 complex and demonstration of antigen 85B on the Mycobacterium leprae cell wall surface.

Authors:  A Rambukkana; P K Das; J D Burggraaf; S Yong; W R Faber; J E Thole; M Harboe
Journal:  Infect Immun       Date:  1992-12       Impact factor: 3.441

10.  Role of the mononuclear phagocyte as an antigen-presenting cell for human gamma delta T cells activated by live Mycobacterium tuberculosis.

Authors:  W H Boom; K A Chervenak; M A Mincek; J J Ellner
Journal:  Infect Immun       Date:  1992-09       Impact factor: 3.441
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