BACKGROUND: As warm ischaemic damage is a major reason for the loss of donor organs, an experimental study was performed in order to evaluate retrograde oxygen persufflation (ROP) as a method to extend the warm ischaemic tolerance of kidneys. METHODS: Kidneys of 32 pigs were exposed to warm ischaemia for 60, 90 or 120 min. Then, 16 kidneys were subjected to ROP for 4 h at 4 degrees C and 16 controls were stored in cold UW-solution, followed by autotransplantation. RESULTS: Only in the group with 60 min warm ischaemic time and ROP did all animals survive the observation period of 7 days. In all other groups some animals died due to anuria. Short-term survivors in these groups had significantly higher creatinine levels. CONCLUSIONS: In this setting, ROP was superior to cold storage when applied after 60 min of warm ischaemia. Clinical evaluation of ROP in the setting of marginal donors and non-heartbeating donation is recommended.
BACKGROUND: As warm ischaemic damage is a major reason for the loss of donor organs, an experimental study was performed in order to evaluate retrograde oxygen persufflation (ROP) as a method to extend the warm ischaemic tolerance of kidneys. METHODS: Kidneys of 32 pigs were exposed to warm ischaemia for 60, 90 or 120 min. Then, 16 kidneys were subjected to ROP for 4 h at 4 degrees C and 16 controls were stored in cold UW-solution, followed by autotransplantation. RESULTS: Only in the group with 60 min warm ischaemic time and ROP did all animals survive the observation period of 7 days. In all other groups some animals died due to anuria. Short-term survivors in these groups had significantly higher creatinine levels. CONCLUSIONS: In this setting, ROP was superior to cold storage when applied after 60 min of warm ischaemia. Clinical evaluation of ROP in the setting of marginal donors and non-heartbeating donation is recommended.
Authors: Thomas M Suszynski; Michael D Rizzari; William E Scott; Linda A Tempelman; Michael J Taylor; Klearchos K Papas Journal: Cryobiology Date: 2012-01-26 Impact factor: 2.487
Authors: W E Scott; B P Weegman; J Ferrer-Fabrega; S A Stein; T Anazawa; V A Kirchner; M D Rizzari; J Stone; S Matsumoto; B E Hammer; A N Balamurugan; L S Kidder; T M Suszynski; E S Avgoustiniatos; S G Stone; L A Tempelman; D E R Sutherland; B J Hering; K K Papas Journal: Transplant Proc Date: 2010 Jul-Aug Impact factor: 1.066
Authors: Stine Lohmann; Marco Eijken; Ulla Møldrup; Bjarne K Møller; James Hunter; Cyril Moers; Rutger J Ploeg; Carla C Baan; Bente Jespersen; Anna Krarup Keller Journal: Transplant Direct Date: 2019-10-08