Literature DB >> 16554728

Removal of contaminating TEM-la beta-lactamase gene from commercial Taq DNA polymerase.

Jae Seok Song1, Jung Hun Lee, Jung-Hyun Lee, Byeong Chul Jeong, Won-Keun Lee, Sang Hee Lee.   

Abstract

This study confirms that Taq DNA polymerase could be contaminated with the blaTEM-1(a) gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.

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Year:  2006        PMID: 16554728

Source DB:  PubMed          Journal:  J Microbiol        ISSN: 1225-8873            Impact factor:   3.422


  5 in total

Review 1.  Call of the wild: antibiotic resistance genes in natural environments.

Authors:  Heather K Allen; Justin Donato; Helena Huimi Wang; Karen A Cloud-Hansen; Julian Davies; Jo Handelsman
Journal:  Nat Rev Microbiol       Date:  2010-03-01       Impact factor: 60.633

2.  First experience of a multicenter external quality assessment of molecular 16S rRNA gene detection in bone and joint infections.

Authors:  Chloé Plouzeau; Pascale Bémer; Anne Sophie Valentin; Geneviève Héry-Arnaud; Didier Tandé; Anne Jolivet-Gougeon; Pascal Vincent; Marie Kempf; Carole Lemarié; Jérôme Guinard; Laurent Bret; Anne Sophie Cognée; Sophie Gibaud; Christophe Burucoa; Stéphane Corvec
Journal:  J Clin Microbiol       Date:  2014-11-19       Impact factor: 5.948

3.  Fast and Accurate Large-Scale Detection of β-Lactamase Genes Conferring Antibiotic Resistance.

Authors:  Jae Jin Lee; Jung Hun Lee; Dae Beom Kwon; Jeong Ho Jeon; Kwang Seung Park; Chang-Ro Lee; Sang Hee Lee
Journal:  Antimicrob Agents Chemother       Date:  2015-07-13       Impact factor: 5.191

4.  Importance of Sequencing To Determine Functional blaTEM Variants.

Authors:  João Pedro Rueda Furlan; Eliana Guedes Stehling; André Pitondo-Silva
Journal:  Antimicrob Agents Chemother       Date:  2017-04-24       Impact factor: 5.191

5.  Plasmid DNA contaminant in molecular reagents.

Authors:  N Wally; M Schneider; J Thannesberger; M T Kastner; T Bakonyi; S Indik; T Rattei; J Bedarf; F Hildebrand; J Law; J Jovel; C Steininger
Journal:  Sci Rep       Date:  2019-02-07       Impact factor: 4.379

  5 in total

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