RATIONALE AND OBJECTIVES: We evaluated the potential of using intravascular magnetic resonance (MR)/radiofrequency (RF) to enhance vascular endothelial growth factor (VEGF) gene therapy of in-stent neointimal hyperplasia. MATERIALS AND METHODS: By using a catheter-based approach, VEGF/lentivirus was locally transferred into 10 (five paired) bilateral femoral-iliac arteries of five hypercholesterolemic pigs, whereas the right arteries were heated up to approximately 41 degrees C by using an intravascular MR/RF system. Then, identical stents were placed immediately into the bilateral VEGF-targeted arteries to create in-stent neointimal hyperplasia. At day 60 after gene/stent interventions, the targeted arteries were harvested for histological correlation. RESULTS: X-Ray angiography-detectable in-stent stenoses were found in three of the arteries treated with VEGF genes only, whereas there were no in-stent stenoses in arteries treated by using MR/RF-heated VEGF genes. Correlative histological examination confirmed a 138% reduction in average thickness of neointimal hyperplasia in VEGF/RF-treated arteries compared with VEGF-only-treated arteries (P < .01). CONCLUSION: We report a potential method of using an intravascular MR/RF heating technique to enhance gene therapy of in-stent restenosis.
RATIONALE AND OBJECTIVES: We evaluated the potential of using intravascular magnetic resonance (MR)/radiofrequency (RF) to enhance vascular endothelial growth factor (VEGF) gene therapy of in-stent neointimal hyperplasia. MATERIALS AND METHODS: By using a catheter-based approach, VEGF/lentivirus was locally transferred into 10 (five paired) bilateral femoral-iliac arteries of five hypercholesterolemicpigs, whereas the right arteries were heated up to approximately 41 degrees C by using an intravascular MR/RF system. Then, identical stents were placed immediately into the bilateral VEGF-targeted arteries to create in-stent neointimal hyperplasia. At day 60 after gene/stent interventions, the targeted arteries were harvested for histological correlation. RESULTS: X-Ray angiography-detectable in-stent stenoses were found in three of the arteries treated with VEGF genes only, whereas there were no in-stent stenoses in arteries treated by using MR/RF-heated VEGF genes. Correlative histological examination confirmed a 138% reduction in average thickness of neointimal hyperplasia in VEGF/RF-treated arteries compared with VEGF-only-treated arteries (P < .01). CONCLUSION: We report a potential method of using an intravascular MR/RF heating technique to enhance gene therapy of in-stent restenosis.
Authors: Darren L Walters; Scott A Harding; Craig R Walsh; Phillip Wong; Eugene Pomerantsev; Ik Kyung Jang Journal: Am J Cardiol Date: 2002-03-01 Impact factor: 2.778
Authors: Marja Hedman; Juha Hartikainen; Mikko Syvänne; Joachim Stjernvall; Antti Hedman; Antti Kivelä; Esko Vanninen; Hanna Mussalo; Esa Kauppila; Sakari Simula; Outi Närvänen; Arto Rantala; Keijo Peuhkurinen; Markku S Nieminen; Markku Laakso; Seppo Ylä-Herttuala Journal: Circulation Date: 2003-05-12 Impact factor: 29.690