Xiao-Fei Zhou1, Qian Wang, Jian-Xin Chu, Ai-Lian Liu. 1. Center for Developmental Biology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
Abstract
AIM: To study the effect of retrorsine on mouse hepatocyte proliferation. METHODS: Mice and rats were treated respectively with two injections of retrorsine (as retrosine-treated group) or saline (as non-treated group) at 2 wk intervals. They received a single injection of carbon tetrachloride (CCl4) 4 wk later. On d 0, 1, 2, 3, 4, 6, 15 after CCl4 administration, the animals were killed and their livers were excised. Hematoxylin and eosin (HE) staining and Ki-67 antibody immunohistochemical analysis of liver samples were used to evaluate the pathological changes and hepatocyte proliferation. RESULTS: In rats treated with retrorsine and CCl4, the liver displayed obvious megalocytosis, proliferation of mild bile duct,small hepatocyte-forming nodule, which were not found in liver samples from non-treated group. However,in mice treated with retrorsine combined with CCl4, the liver displayed hepatocyte degeneration and necrosis in perivenous areas.There was no obvious difference between retrorsine-treated group and non-treated group. Ki-67 immunohistochemical analysis showed that in rats treated with retrorsine, the positive hepatocytes mainly found in small hepatocyte nodules, were obviously less than those in non-treated group. The mice treated with retrorsine showed that the number of Ki-67 positive hepatocytes was very high and more than that in non-treated group. CONCLUSION: Retrorsine has no effect on mouse hepatocyte proliferation.
AIM: To study the effect of retrorsine on mouse hepatocyte proliferation. METHODS:Mice and rats were treated respectively with two injections of retrorsine (as retrosine-treated group) or saline (as non-treated group) at 2 wk intervals. They received a single injection of carbon tetrachloride (CCl4) 4 wk later. On d 0, 1, 2, 3, 4, 6, 15 after CCl4 administration, the animals were killed and their livers were excised. Hematoxylin and eosin (HE) staining and Ki-67 antibody immunohistochemical analysis of liver samples were used to evaluate the pathological changes and hepatocyte proliferation. RESULTS: In rats treated with retrorsine and CCl4, the liver displayed obvious megalocytosis, proliferation of mild bile duct,small hepatocyte-forming nodule, which were not found in liver samples from non-treated group. However,in mice treated with retrorsine combined with CCl4, the liver displayed hepatocyte degeneration and necrosis in perivenous areas.There was no obvious difference between retrorsine-treated group and non-treated group. Ki-67 immunohistochemical analysis showed that in rats treated with retrorsine, the positive hepatocytes mainly found in small hepatocyte nodules, were obviously less than those in non-treated group. The mice treated with retrorsine showed that the number of Ki-67 positive hepatocytes was very high and more than that in non-treated group. CONCLUSION:Retrorsine has no effect on mouse hepatocyte proliferation.
Authors: P Birner; M Ritzi; C Musahl; R Knippers; J Gerdes; T Voigtländer; H Budka; J A Hainfellner Journal: Am J Pathol Date: 2001-06 Impact factor: 4.307
Authors: E Laconi; R Oren; D K Mukhopadhyay; E Hurston; S Laconi; P Pani; M D Dabeva; D A Shafritz Journal: Am J Pathol Date: 1998-07 Impact factor: 4.307
Authors: Eleftherios Michailidis; Koen Vercauteren; Liliana Mancio-Silva; Linda Andrus; Cyprien Jahan; Inna Ricardo-Lax; Chenhui Zou; Mohammad Kabbani; Paul Park; Corrine Quirk; Christina Pyrgaki; Brandon Razooky; Lieven Verhoye; Irene Zoluthkin; Wei-Yu Lu; Stuart J Forbes; Luis Chiriboga; Neil D Theise; Roland W Herzog; Hiroshi Suemizu; William M Schneider; Amir Shlomai; Philip Meuleman; Sangeeta N Bhatia; Charles M Rice; Ype P de Jong Journal: Proc Natl Acad Sci U S A Date: 2020-01-08 Impact factor: 11.205