| Literature DB >> 16551073 |
Wai Cheu Lai1, Neville McLean, Axel Gansmüller, Michiel A Verhoeven, Gian Carlo Antonioli, Marina Carravetta, Luminita Duma, Petra H M Bovee-Geurts, Ole G Johannessen, Huub J M de Groot, Johan Lugtenburg, Lyndon Emsley, Steven P Brown, Richard C D Brown, Willem J DeGrip, Malcolm H Levitt.
Abstract
A new double-quantum solid-state NMR pulse sequence is presented and used to measure one-bond 13C-13C J-couplings in a set of 13C2-labeled rhodopsin isotopomers. The measured J-couplings reveal a perturbation of the electronic structure at the terminus of the conjugated chain but show no evidence for protein-induced electronic perturbation near the C11-C12 isomerization site. This work establishes NMR methodology for measuring accurate 1JCC values in noncrystalline macromolecules and shows that the measured J-couplings may reveal local electronic perturbations of mechanistic significance.Entities:
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Year: 2006 PMID： 16551073 DOI： 10.1021/ja0581604
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419