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Literature DB >> 16547462

Structure and oligomeric state of human transcription factor TFIIE.

Anass Jawhari¹, Muriel Uhring, Sacha De Carlo, Corinne Crucifix, Guiseppe Tocchini-Valentini, Dino Moras, Patrick Schultz, Arnaud Poterszman.

Abstract

The general RNA polymerase II transcription factor TFIIE, which is composed of two subunits, has essential roles in both transcription initiation and promoter escape. Electron microscopy analysis of negatively stained human TFIIE showed a large proportion of alpha/beta heterodimers as well as a small proportion of tetramers. Analytical ultracentrifugation, chemical crosslinking, pulldown experiments and cryo-electron microscopy confirmed that TFIIE is a alpha/beta heterodimer in solution. Three-dimensional envelopes of the alpha/beta particles showed an elongated structure composed of three distinct modules. Finally, a model for the quaternary architecture of the complex is proposed that provides a structural framework to discuss the function of TFIIE in the context of RNA polymerase II transcription initiation.

Entities: Gene Species

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Year: 2006 PMID： 16547462 PMCID： PMC1479554 DOI： 10.1038/sj.embor.7400663

Source DB: PubMed Journal: EMBO Rep ISSN： 1469-221X Impact factor: 8.807

26 in total

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4. Cryo-negative staining reduces electron-beam sensitivity of vitrified biological particles.

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5. Cryo-negative staining reveals conformational flexibility within yeast RNA polymerase I.

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6. Dissecting the interaction network of multiprotein complexes by pairwise coexpression of subunits in E. coli.

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Authors: Yoshiyuki Itoh; Satoru Unzai; Mamoru Sato; Aritaka Nagadoi; Masahiko Okuda; Yoshifumi Nishimura; Satoko Akashi
Journal: Proteins Date: 2005-11-15

8. Crystallization properties and structure of Panulirus interruptus haemocyanin.

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9. Structure of the central core domain of TFIIEbeta with a novel double-stranded DNA-binding surface.

Authors: M Okuda; Y Watanabe; H Okamura; F Hanaoka; Y Ohkuma; Y Nishimura
Journal: EMBO J Date: 2000-03-15 Impact factor: 11.598

10. The Rpb9 subunit of RNA polymerase II binds transcription factor TFIIE and interferes with the SAGA and elongator histone acetyltransferases.

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5. Structural characterization of human general transcription factor TFIIF in solution.

Authors: Satoko Akashi; Shinjiro Nagakura; Seiji Yamamoto; Masahiko Okuda; Yoshiaki Ohkuma; Yoshifumi Nishimura
Journal: Protein Sci Date: 2008-01-24 Impact factor: 6.725

6. Structural basis for HIV-1 DNA integration in the human genome, role of the LEDGF/P75 cofactor.

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Journal: EMBO J Date: 2009-02-19 Impact factor: 11.598