E Scarr1, L Gray, D Keriakous, P J Robinson, B Dean. 1. Rebecca L. Cooper Research Laboratories, The Mental Health Research Institute of Victoria, Parkville, Victoria, Australia. elscarr@unimelb.edu.au
Abstract
OBJECTIVE: In order to identify whether the mechanisms associated with neurotransmitter release are involved in the pathologies of bipolar disorder and schizophrenia, levels of presynaptic [synaptosomal-associated protein-25 (SNAP-25), syntaxin, synaptophysin, vesicle-associated membrane protein, dynamin I] and structural (neuronal cell adhesion molecule and alpha-synuclein) neuronal markers were measured in Brodmann's area 9 obtained postmortem from eight subjects with bipolar I disorder (BPDI), 20 with schizophrenia and 20 controls. METHODS: Determinations of protein levels were carried out using Western blot techniques with specific antibodies. Levels of mRNA were measured using real-time polymerase chain reaction. RESULTS: In BPDI, levels of SNAP-25 (p < 0.01) and synaptophysin (p < 0.05) increased. There were no changes in schizophrenia or any other changes in BPDI. Levels of mRNA for SNAP-25 were decreased in BPDI (p < 0.05). CONCLUSION: Changes in SNAP-25 and synaptophysin in BPDI suggest that changes in specific neuronal functions could be linked to the pathology of the disorder.
OBJECTIVE: In order to identify whether the mechanisms associated with neurotransmitter release are involved in the pathologies of bipolar disorder and schizophrenia, levels of presynaptic [synaptosomal-associated protein-25 (SNAP-25), syntaxin, synaptophysin, vesicle-associated membrane protein, dynamin I] and structural (neuronal cell adhesion molecule and alpha-synuclein) neuronal markers were measured in Brodmann's area 9 obtained postmortem from eight subjects with bipolar I disorder (BPDI), 20 with schizophrenia and 20 controls. METHODS: Determinations of protein levels were carried out using Western blot techniques with specific antibodies. Levels of mRNA were measured using real-time polymerase chain reaction. RESULTS: In BPDI, levels of SNAP-25 (p < 0.01) and synaptophysin (p < 0.05) increased. There were no changes in schizophrenia or any other changes in BPDI. Levels of mRNA for SNAP-25 were decreased in BPDI (p < 0.05). CONCLUSION: Changes in SNAP-25 and synaptophysin in BPDI suggest that changes in specific neuronal functions could be linked to the pathology of the disorder.
Authors: V E Golimbet; M V Alfimova; I K Gritsenko; T V Lezheiko; O M Lavrushina; L I Abramova; V G Kaleda; A N Barkhatova; A V Sokolov; R P Ebstein Journal: Neurosci Behav Physiol Date: 2010-05
Authors: Peter M Thompson; Dianne A Cruz; Elizabeth A Fucich; Dianna Y Olukotun; Masami Takahashi; Makoto Itakura Journal: Mol Neuropsychiatry Date: 2015-12-01
Authors: A Terracciano; S Sanna; M Uda; B Deiana; G Usala; F Busonero; A Maschio; M Scally; N Patriciu; W-M Chen; M A Distel; E P Slagboom; D I Boomsma; S Villafuerte; E Sliwerska; M Burmeister; N Amin; A C J W Janssens; C M van Duijn; D Schlessinger; G R Abecasis; P T Costa Journal: Mol Psychiatry Date: 2008-10-28 Impact factor: 15.992
Authors: Falk W Lohoff; Andrew E Weller; Paul J Bloch; Aleksandra H Nall; Thomas N Ferraro; Wade H Berrettini Journal: J Neural Transm (Vienna) Date: 2008-07-30 Impact factor: 3.575
Authors: Akin Oni-Orisan; Lars V Kristiansen; Vahram Haroutunian; James H Meador-Woodruff; Robert E McCullumsmith Journal: Biol Psychiatry Date: 2007-12-26 Impact factor: 13.382