J W Dieker1, C C Van Bavel, G Riemekasten, J H Berden, J van der Vlag. 1. Nephrology Research Laboratory, Nijmegen Centre for Molecular Life Sciences, Division of Nephrology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
Abstract
OBJECTIVES: To evaluate the binding of lupus-derived autoantibodies, double-stranded DNA and nucleosomes to the positively charged C-terminal SmD1(residues 83-119) peptide and the full-length SmD protein. METHODS: The binding of lupus-derived monoclonal antibodies, sera from patients with systemic lupus erythematosus, rheumatoid arthritis and systemic sclerosis, dsDNA and nucleosomes to the SmD1(83-119) peptide or the full-length SmD protein was determined using different ELISA methods. RESULTS: Monoclonal anti-dsDNA antibodies and the serum of patients with systemic lupus erythematosus that are positive for anti-dsDNA antibodies react with the SmD1(83-119) peptide in ELISA. However, DNaseI treatment of the blocking reagents leads to a decreased reactivity. Purified dsDNA and nucleosomes bind to the SmD1 peptide but not to the full-length SmD protein. CONCLUSIONS: The SmD1(83-119) peptide is able to bind dsDNA and nucleosomes, and dsDNA or nucleosomes in applied reagents lead to an apparent reactivity of anti-dsDNA, anti-histone or nucleosome-specific antibodies with the SmD1(83-119) peptide in ELISA.
OBJECTIVES: To evaluate the binding of lupus-derived autoantibodies, double-stranded DNA and nucleosomes to the positively charged C-terminal SmD1(residues 83-119) peptide and the full-length SmD protein. METHODS: The binding of lupus-derived monoclonal antibodies, sera from patients with systemic lupus erythematosus, rheumatoid arthritis and systemic sclerosis, dsDNA and nucleosomes to the SmD1(83-119) peptide or the full-length SmD protein was determined using different ELISA methods. RESULTS: Monoclonal anti-dsDNA antibodies and the serum of patients with systemic lupus erythematosus that are positive for anti-dsDNA antibodies react with the SmD1(83-119) peptide in ELISA. However, DNaseI treatment of the blocking reagents leads to a decreased reactivity. Purified dsDNA and nucleosomes bind to the SmD1 peptide but not to the full-length SmD protein. CONCLUSIONS: The SmD1(83-119) peptide is able to bind dsDNA and nucleosomes, and dsDNA or nucleosomes in applied reagents lead to an apparent reactivity of anti-dsDNA, anti-histone or nucleosome-specific antibodies with the SmD1(83-119) peptide in ELISA.
Authors: Jürgen W Dieker; Yong-Jiang Sun; Cor W Jacobs; Chaim Putterman; Marc Monestier; Sylviane Muller; Johan van der Vlag; Jo H Berden Journal: J Immunol Methods Date: 2004-12-02 Impact factor: 2.303
Authors: G Riemekasten; J Marell; G Trebeljahr; R Klein; G Hausdorf; T Häupl; J Schneider-Mergener; G R Burmester; F Hiepe Journal: J Clin Invest Date: 1998-08-15 Impact factor: 14.808
Authors: Gabriela Riemekasten; Dirk Langnickel; Fanny M Ebling; George Karpouzas; Jatinderpal Kalsi; Gunda Herberth; Betty P Tsao; Peter Henklein; Sven Langer; Gerd-R Burmester; Andreas Radbruch; Falk Hiepe; Bevra H Hahn Journal: Arthritis Rheum Date: 2003-02
Authors: C Kramers; M N Hylkema; M C van Bruggen; R van de Lagemaat; H B Dijkman; K J Assmann; R J Smeenk; J H Berden Journal: J Clin Invest Date: 1994-08 Impact factor: 14.808
Authors: M Reichlin; A Martin; E Taylor-Albert; K Tsuzaka; W Zhang; M W Reichlin; E Koren; F M Ebling; B Tsao; B H Hahn Journal: J Clin Invest Date: 1994-01 Impact factor: 14.808