Literature DB >> 16535756

Expression of Barley Endopeptidase B in Trichoderma reesei.

R Saarelainen, A Mantyla, H Nevalainen, P Suominen.   

Abstract

The gene for barley endopeptidase B (EPB) has been expressed in the filamentous fungus Trichoderma reesei from the cbh1 promoter. The EPB signal sequence allowed secretion of over 90% of the recombinant protein. Yields reached about 500 mg of immunoreactive protein per liter and exceeded values for any other protein derived from a higher eukaryotic organism produced in T. reesei.

Entities:  

Year:  1997        PMID： 16535756      PMCID： PMC1389312          DOI： 10.1128/aem.63.12.4938-4940.1997

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

1.  High frequency one-step gene replacement in Trichoderma reesei. II. Effects of deletions of individual cellulase genes.

Authors:  P L Suominen; A L Mäntylä; T Karhunen; S Hakola; H Nevalainen
Journal:  Mol Gen Genet       Date:  1993-12

2.  High frequency one-step gene replacement in Trichoderma reesei. I. Endoglucanase I overproduction.

Authors:  T Karhunen; A Mäntylä; K M Nevalainen; P L Suominen
Journal:  Mol Gen Genet       Date:  1993-12

3.  Improved production of Trichoderma harzianum endochitinase by expression in Trichoderma reesei.

Authors:  E Margolles-Clark; C K Hayes; G E Harman; M Penttilä
Journal:  Appl Environ Microbiol       Date:  1996-06       Impact factor: 4.792

4.  A major gibberellic Acid-induced barley aleurone cysteine proteinase which digests hordein : purification and characterization.

Authors:  S M Koehler; T H Ho
Journal:  Plant Physiol       Date:  1990-09       Impact factor: 8.340

5.  Purification and characterization of gibberellic Acid-induced cysteine endoproteases in barley aleurone layers.

Authors:  S Koehler; T H Ho
Journal:  Plant Physiol       Date:  1988-05       Impact factor: 8.340

6.  Overexpression of the Aspergillus niger pH 2.5 acid phosphatase gene in a heterologous host Trichoderma reesei.

Authors:  A Miettinen-Oinonen; T Torkkeli; M Paloheimo; H Nevalainen
Journal:  J Biotechnol       Date:  1997-10-02       Impact factor: 3.307

7.  Expression and Secretion of Barley Cysteine Endopeptidase B and Cellobiohydrolase I in Trichoderma reesei.

Authors:  M Nykanen; R Saarelainen; M Raudaskoski; K Nevalainen; A Mikkonen
Journal:  Appl Environ Microbiol       Date:  1997-12       Impact factor: 4.792

8.  Transformation of Trichoderma reesei with the Hormoconis resinae glucoamylase P (gamP) gene: production of a heterologous glucoamylase by Trichoderma reesei.

Authors:  V V Joutsjoki; T K Torkkeli; K M Nevalainen
Journal:  Curr Genet       Date:  1993-09       Impact factor: 3.886

9.  Efficient production of antibody fragments by the filamentous fungus Trichoderma reesei.

Authors:  E Nyyssönen; M Penttilä; A Harkki; A Saloheimo; J K Knowles; S Keränen
Journal:  Biotechnology (N Y)       Date:  1993-05

10.  Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans.

Authors:  J M Kelly; M J Hynes
Journal:  EMBO J       Date:  1985-02       Impact factor: 11.598
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  3 in total

1.  Expression and Secretion of Barley Cysteine Endopeptidase B and Cellobiohydrolase I in Trichoderma reesei.

Authors:  M Nykanen; R Saarelainen; M Raudaskoski; K Nevalainen; A Mikkonen
Journal:  Appl Environ Microbiol       Date:  1997-12       Impact factor: 4.792

Review 2.  Making recombinant proteins in filamentous fungi- are we expecting too much?

Authors:  Helena Nevalainen; Robyn Peterson
Journal:  Front Microbiol       Date:  2014-02-27       Impact factor: 5.640

3.  A versatile system for fast screening and isolation of Trichoderma reesei cellulase hyperproducers based on DsRed and fluorescence-assisted cell sorting.

Authors:  Fei Gao; Zhenzhen Hao; Xianhua Sun; Lina Qin; Tong Zhao; Weiquan Liu; Huiying Luo; Bin Yao; Xiaoyun Su
Journal:  Biotechnol Biofuels       Date:  2018-09-24       Impact factor: 6.040
  3 in total

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