| Literature DB >> 16535740 |
I Loubens, W S Chilton, P Dion.
Abstract
Agrobacterium tumefaciens C58 grown on acidic medium containing glucose and solidified with bacteriological agar expressed a virB::lacZ fusion. No expression of this fusion was observed on a similar medium which was solidified with purified agarose. The fraction from bacteriological agar which was responsible for vir gene induction was extracted with methanol and partially purified by preparative thin-layer chromatography.Entities:
Year: 1997 PMID: 16535740 PMCID: PMC1389296 DOI: 10.1128/aem.63.11.4578-4580.1997
Source DB: PubMed Journal: Appl Environ Microbiol ISSN: 0099-2240 Impact factor: 4.792