Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Purification and characterization of two extracellular alkaline phosphatases from a psychrophilic arthrobacter isolate.

Literature DB >> 16535659

Purification and characterization of two extracellular alkaline phosphatases from a psychrophilic arthrobacter isolate.

Abstract

Two extracellular, heat-labile alkaline phosphatases were purified from a psychrophilic Arthrobacter isolate, D10. The enzymes were active over different pH ranges, used distinct substrates, and had different kinetic properties. Each enzyme reacted specifically to its own antibody during immunoblot analysis. One had both monophosphatase and diesterase activities.

Entities: Gene

Year: 1997 PMID： 16535659 PMCID： PMC1389214 DOI： 10.1128/aem.63.7.2928-2931.1997

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

Keyword Cloud
References

14 in total

1. Studies on plant amylases: The effect of starch concentration upon the velocity of hydrolysis by the amylase of germinated barley.

Authors: C S Hanes
Journal: Biochem J Date: 1932 Impact factor: 3.857

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

3. Studies on the extracellular alkaline phosphatase of Micrococcus sodonensis. I. Isolation and characterization.

Authors: R H Glew; E C Heath
Journal: J Biol Chem Date: 1971-03-25 Impact factor: 5.157

4. Long-term immunogenicity and efficacy of hepatitis B vaccine in infants born to HBeAg-positive HBsAg-carrier mothers.

Authors: K J Lo; S D Lee; Y T Tsai; T C Wu; C Y Chan; G H Chen; C L Yeh
Journal: Hepatology Date: 1988 Nov-Dec Impact factor: 17.425

5. A malachite green procedure for orthophosphate determination and its use in alkaline phosphatase-based enzyme immunoassay.

Authors: A A Baykov; O A Evtushenko; S M Avaeva
Journal: Anal Biochem Date: 1988-06 Impact factor: 3.365

6. Extracellular Ca2(+)-dependent inducible alkaline phosphatase from extremely halophilic archaebacterium Haloarcula marismortui.

Authors: S Goldman; K Hecht; H Eisenberg; M Mevarech
Journal: J Bacteriol Date: 1990-12 Impact factor: 3.490

Purification and characterization of two extracellular alkaline phosphatases from a psychrophilic arthrobacter isolate.

1. Studies on plant amylases: The effect of starch concentration upon the velocity of hydrolysis by the amylase of germinated barley.

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

3. Studies on the extracellular alkaline phosphatase of Micrococcus sodonensis. I. Isolation and characterization.

4. Long-term immunogenicity and efficacy of hepatitis B vaccine in infants born to HBeAg-positive HBsAg-carrier mothers.

5. A malachite green procedure for orthophosphate determination and its use in alkaline phosphatase-based enzyme immunoassay.

6. Extracellular Ca2(+)-dependent inducible alkaline phosphatase from extremely halophilic archaebacterium Haloarcula marismortui.

7. Staining technique for phosphatases in polyacrylamide gels.

8. Isolation and properties of a small manganese-ion-stimulated bacterial alkaline phosphatase.

9. Characterization of a psychrotrophic Arthrobacter gene and its cold-active beta-galactosidase.

10. Characterization of psychrotrophic microorganisms producing beta-galactosidase activities.