Literature DB >> 16535597

A Change in a Single Midgut Receptor in the Diamondback Moth (Plutella xylostella) Is Only in Part Responsible for Field Resistance to Bacillus thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai.

D J Wright, M Iqbal, F Granero, J Ferre.   

Abstract

A population (SERD3) of the diamondback moth (Plutella xylostella L.) with field-evolved resistance to Bacillus thuringiensis subsp. kurstaki HD-1 (Dipel) and B. thuringiensis subsp. aizawai (Florbac) was collected. Laboratory-based selection of two subpopulations of SERD3 with B. thuringiensis subsp. kurstaki (Btk-Sel) or B. thuringiensis subsp. aizawai (Bta-Sel) increased resistance to the selecting agent with little apparent cross-resistance. This result suggested the presence of independent resistance mechanisms. Reversal of resistance to B. thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai was observed in the unselected SERD3 subpopulation. Binding to midgut brush border membrane vesicles was examined for insecticidal crystal proteins specific to B. thuringiensis subsp. kurstaki (Cry1Ac), B. thuringiensis subsp. aizawai (Cry1Ca), or both (Cry1Aa and Cry1Ab). In the unselected SERD3 subpopulation (ca. 50- and 30-fold resistance to B. thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai), specific binding of Cry1Aa, Cry1Ac, and Cry1Ca was similar to that for a susceptible population (ROTH), but binding of Cry1Ab was minimal. The Btk-Sel (ca. 600-and 60-fold resistance to B. thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai) and Bta-Sel (ca. 80-and 300-fold resistance to B. thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai) subpopulations also showed reduced binding to Cry1Ab. Binding of Cry1Ca was not affected in the Bta-Sel subpopulation. The results suggest that reduced binding of Cry1Ab can partly explain resistance to B. thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai. However, the binding of Cry1Aa, Cry1Ac, and Cry1Ca and the lack of cross-resistance between the Btk-Sel and Bta-Sel subpopulations also suggest that additional resistance mechanisms are present.

Entities:  

Year:  1997        PMID： 16535597      PMCID： PMC1389152          DOI： 10.1128/aem.63.5.1814-1819.1997

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  27 in total

1.  Comparative analysis of the individual protoxin components in P1 crystals of Bacillus thuringiensis subsp. kurstaki isolates NRD-12 and HD-1.

Authors:  L Masson; G Préfontaine; L Péloquin; P C Lau; R Brousseau
Journal:  Biochem J       Date:  1990-07-15       Impact factor: 3.857

2.  The toxicity of two Bacillus thuringiensis delta-endotoxins to gypsy moth larvae is inversely related to the affinity of binding sites on midgut brush border membranes for the toxins.

Authors:  M G Wolfersberger
Journal:  Experientia       Date:  1990-05-15

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Receptors on the brush border membrane of the insect midgut as determinants of the specificity of Bacillus thuringiensis delta-endotoxins.

Authors:  J Van Rie; S Jansens; H Höfte; D Degheele; H Van Mellaert
Journal:  Appl Environ Microbiol       Date:  1990-05       Impact factor: 4.792

5.  Resistance to the Bacillus thuringiensis bioinsecticide in a field population of Plutella xylostella is due to a change in a midgut membrane receptor.

Authors:  J Ferré; M D Real; J Van Rie; S Jansens; M Peferoen
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-15       Impact factor: 11.205

6.  Ligand: a versatile computerized approach for characterization of ligand-binding systems.

Authors:  P J Munson; D Rodbard
Journal:  Anal Biochem       Date:  1980-09-01       Impact factor: 3.365

7.  Broad-spectrum resistance to Bacillus thuringiensis toxins in Heliothis virescens.

Authors:  F Gould; A Martinez-Ramirez; A Anderson; J Ferre; F J Silva; W J Moar
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-01       Impact factor: 11.205

Review 8.  Insecticidal crystal proteins of Bacillus thuringiensis.

Authors:  H Höfte; H R Whiteley
Journal:  Microbiol Rev       Date:  1989-06

9.  Mechanism of insect resistance to the microbial insecticide Bacillus thuringiensis.

Authors:  J Van Rie; W H McGaughey; D E Johnson; B D Barnett; H Van Mellaert
Journal:  Science       Date:  1990-01-05       Impact factor: 47.728

10.  Binding of the delta endotoxin from Bacillus thuringiensis to brush-border membrane vesicles of the cabbage butterfly (Pieris brassicae).

Authors:  C Hofmann; P Lüthy; R Hütter; V Pliska
Journal:  Eur J Biochem       Date:  1988-04-05
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  26 in total

1.  Role of bacillus thuringiensis toxin domains in toxicity and receptor binding in the diamondback moth

Authors: 
Journal:  Appl Environ Microbiol       Date:  1999-05       Impact factor: 4.792

2.  Association of Cry1Ac toxin resistance in Helicoverpa zea (Boddie) with increased alkaline phosphatase levels in the midgut lumen.

Authors:  Silvia Caccia; William J Moar; Jayadevi Chandrashekhar; Cris Oppert; Konasale J Anilkumar; Juan Luis Jurat-Fuentes; Juan Ferré
Journal:  Appl Environ Microbiol       Date:  2012-06-08       Impact factor: 4.792

3.  Interactions Between Bt-Bioinsecticides and Podisus nigrispinus (Dallas) (Hemiptera: Pentatomidae), a Predator of Plutella xylostella (L.) (Lepidoptera: Plutellidae).

Authors:  G O Magalhães; A M Vacari; C P DE Bortoli; A F Pomari; S A DE Bortoli; R A Polanczyk
Journal:  Neotrop Entomol       Date:  2015-08-16       Impact factor: 1.434

4.  Common receptor for Bacillus thuringiensis toxins Cry1Ac, Cry1Fa, and Cry1Ja in Helicoverpa armigera, Helicoverpa zea, and Spodoptera exigua.

Authors:  Carmen Sara Hernández; Juan Ferré
Journal:  Appl Environ Microbiol       Date:  2005-09       Impact factor: 4.792

Review 5.  Role of receptors in Bacillus thuringiensis crystal toxin activity.

Authors:  Craig R Pigott; David J Ellar
Journal:  Microbiol Mol Biol Rev       Date:  2007-06       Impact factor: 11.056

6.  Bacillus thuringiensis Cry1Ac toxin-binding and pore-forming activity in brush border membrane vesicles prepared from anterior and posterior midgut regions of lepidopteran larvae.

Authors:  Ana Rodrigo-Simón; Silvia Caccia; Juan Ferré
Journal:  Appl Environ Microbiol       Date:  2008-01-25       Impact factor: 4.792

7.  Cytotoxic activity of Bacillus thuringiensis Cry proteins on mammalian cells transfected with cadherin-like Cry receptor gene of Bombyx mori (silkworm).

Authors:  Yoko Tsuda; Fumiki Nakatani; Keiko Hashimoto; Satoshi Ikawa; Chikako Matsuura; Takashi Fukada; Kenji Sugimoto; Michio Himeno
Journal:  Biochem J       Date:  2003-02-01       Impact factor: 3.857

8.  Genetic and biochemical characterization of field-evolved resistance to Bacillus thuringiensis toxin Cry1Ac in the diamondback moth, Plutella xylostella.

Authors:  Ali H Sayyed; Ben Raymond; M Sales Ibiza-Palacios; Baltasar Escriche; Denis J Wright
Journal:  Appl Environ Microbiol       Date:  2004-12       Impact factor: 4.792

Review 9.  Bacillus thuringiensis and its pesticidal crystal proteins.

Authors:  E Schnepf; N Crickmore; J Van Rie; D Lereclus; J Baum; J Feitelson; D R Zeigler; D H Dean
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

10.  Binding site alteration is responsible for field-isolated resistance to Bacillus thuringiensis Cry2A insecticidal proteins in two Helicoverpa species.

Authors:  Silvia Caccia; Carmen Sara Hernández-Rodríguez; Rod J Mahon; Sharon Downes; William James; Nadine Bautsoens; Jeroen Van Rie; Juan Ferré
Journal:  PLoS One       Date:  2010-04-01       Impact factor: 3.240
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