AIM: To determine whether neutrophil depletion and Kupffer cell inhibition might combine their protective effects to decrease the severity of acute pancreatitis. METHODS: Mice had cerulein administration to induce acute pancreatitis and were pretreated with either anti-mouse neutrophil serum or gadolinium chloride (GdCl3) to prevent Kupffer cell activation, or both treatments. Injury was assessed in pancreas and lungs. Myeloperoxidases (MPO) assessed neutrophil infiltration. Interleukin-6 (IL-6) and IL-10 were measured in serum, pancreas, lungs and liver. RESULTS: In mice with acute pancreatitis, neutrophil depletion reduced the severity of pancreatitis and pancreatitis-associated lung injury. Kupffer cell inactivation by GdCl3 had less protective effect, although IL-6 and IL-10 concentrations were significantly decreased. The protective treatment brought by neutrophil depletion was not enhanced by Kupffer cell inactivation and both treatments did not combine their protective effects. CONCLUSION: Our results confirm the role of activated neutrophils in aggravating organ injury in acute pancreatitis while the role of Kupffer cell activation is less obvious.
AIM: To determine whether neutrophil depletion and Kupffer cell inhibition might combine their protective effects to decrease the severity of acute pancreatitis. METHODS:Mice had cerulein administration to induce acute pancreatitis and were pretreated with either anti-mouse neutrophil serum or gadolinium chloride (GdCl3) to prevent Kupffer cell activation, or both treatments. Injury was assessed in pancreas and lungs. Myeloperoxidases (MPO) assessed neutrophil infiltration. Interleukin-6 (IL-6) and IL-10 were measured in serum, pancreas, lungs and liver. RESULTS: In mice with acute pancreatitis, neutrophil depletion reduced the severity of pancreatitis and pancreatitis-associated lung injury. Kupffer cell inactivation by GdCl3 had less protective effect, although IL-6 and IL-10 concentrations were significantly decreased. The protective treatment brought by neutrophil depletion was not enhanced by Kupffer cell inactivation and both treatments did not combine their protective effects. CONCLUSION: Our results confirm the role of activated neutrophils in aggravating organ injury in acute pancreatitis while the role of Kupffer cell activation is less obvious.
Authors: J Devière; O Le Moine; J L Van Laethem; P Eisendrath; A Ghilain; N Severs; M Cohard Journal: Gastroenterology Date: 2001-02 Impact factor: 22.682
Authors: Anna S Gukovskaya; Eva Vaquero; Vjekoslav Zaninovic; Fred S Gorelick; Aldons J Lusis; Marie-Luise Brennan; Steven Holland; Stephen J Pandol Journal: Gastroenterology Date: 2002-04 Impact factor: 22.682
Authors: Jean-Louis Frossard; Antoine Hadengue; Laurent Spahr; Philippe Morel; Catherine M Pastor Journal: Crit Care Med Date: 2002-07 Impact factor: 7.598
Authors: Michel M Murr; Jun Yang; Adam Fier; Scott F Gallagher; Gay Carter; William R Gower; James G Norman Journal: J Gastrointest Surg Date: 2003-01 Impact factor: 3.452
Authors: Andreas M Lenz; Mark Fairweather; James C Peyton; Sarah A Gardner; William G Cheadle Journal: Inflamm Res Date: 2010-10-26 Impact factor: 4.575
Authors: Zilvinas Dambrauskas; Nathalia Giese; Antanas Gulbinas; Thomas Giese; Pascal O Berberat; Juozas Pundzius; Giedrius Barauskas; Helmut Friess Journal: World J Gastroenterol Date: 2010-04-21 Impact factor: 5.742