BACKGROUND & AIMS: Few genes that regulate intestinal epithelium development, homeostasis, or function are known. We reasoned that potential candidate regulators of these processes could be identified based on their activation during intestinal epithelium development and their subsequent specific and restricted expression. METHODS: Genes were identified by differential display and microarray analyses, further selected according to sequence and UniGene expression profiles, and analyzed by RNA in situ hybridization of mouse fetal and adult intestines and in intestinal polyp tissue. RESULTS: Five genes with unknown physiological function predominantly or exclusively expressed in the intestinal epithelium were identified. Their expression is activated at distinct times during intestinal development and maturation and is maintained in highly specific, spatially distinct patterns in the adult intestinal epithelium. Two of the genes were up-regulated in intestinal tumors, 1 was down-regulated, and 2 were apparently unaltered. CONCLUSIONS: Based on sequence and expression, the identified genes represent good candidates for regulators of intestinal epithelium integrity or function. Their expression patterns suggest a morphologically not obvious molecular regionalization of the intestinal epithelium along the crypt villus axis. This approach should be an efficient means to identify novel genes required for intestinal epithelium homeostasis and function.
BACKGROUND & AIMS: Few genes that regulate intestinal epithelium development, homeostasis, or function are known. We reasoned that potential candidate regulators of these processes could be identified based on their activation during intestinal epithelium development and their subsequent specific and restricted expression. METHODS: Genes were identified by differential display and microarray analyses, further selected according to sequence and UniGene expression profiles, and analyzed by RNA in situ hybridization of mouse fetal and adult intestines and in intestinal polyp tissue. RESULTS: Five genes with unknown physiological function predominantly or exclusively expressed in the intestinal epithelium were identified. Their expression is activated at distinct times during intestinal development and maturation and is maintained in highly specific, spatially distinct patterns in the adult intestinal epithelium. Two of the genes were up-regulated in intestinal tumors, 1 was down-regulated, and 2 were apparently unaltered. CONCLUSIONS: Based on sequence and expression, the identified genes represent good candidates for regulators of intestinal epithelium integrity or function. Their expression patterns suggest a morphologically not obvious molecular regionalization of the intestinal epithelium along the crypt villus axis. This approach should be an efficient means to identify novel genes required for intestinal epithelium homeostasis and function.
Authors: Jay V Patankar; Prakash G Chandak; Sascha Obrowsky; Thomas Pfeifer; Clemens Diwoky; Andreas Uellen; Wolfgang Sattler; Rudolf Stollberger; Gerald Hoefler; Akos Heinemann; Michele Battle; Stephen Duncan; Dagmar Kratky; Sanja Levak-Frank Journal: Am J Physiol Endocrinol Metab Date: 2010-12-21 Impact factor: 4.310
Authors: Ulrich C Klostermeier; Matthias Barann; Michael Wittig; Robert Häsler; Andre Franke; Olga Gavrilova; Benjamin Kreck; Christian Sina; Markus B Schilhabel; Stefan Schreiber; Philip Rosenstiel Journal: BMC Genomics Date: 2011-06-10 Impact factor: 3.969
Authors: Tuomas Kaprio; Jaana Hagström; Harri Mustonen; Selja Koskensalo; Leif C Andersson; Caj Haglund Journal: PLoS One Date: 2014-10-08 Impact factor: 3.240