| Literature DB >> 16530357 |
Lénia Ferrão-Beck1, Regina Cardoso, Pilar María Muñoz, María Jesús de Miguel, David Albert, Ana Cristina Ferreira, Clara María Marín, Martine Thiébaud, Isabelle Jacques, Maggy Grayon, Michel S Zygmunt, Bruno Garin-Bastuji, Jose María Blasco, Maria Inácia Sá.
Abstract
Swine brucellosis is caused by the biovars 1, 2 and 3 of Brucella suis the identification of which up to now relies on microbiological tests lacking adequate specificity together with time consuming and expensive molecular procedures. Based on sequence variation of the omp2b gene, we have developed a four primer set multiplex PCR assay that was tested for polymorphism analysis of B. suis biovars causing brucellosis in swine. The assay exploits the single nucleotide polymorphisms found in omp2b gene of B. suis reference biovars which are conserved in 43 B. suis field isolates from different geographic origins and hosts. Three specific amplification patterns (S1, S2 and S3) were obtained for reference strains of B. suis biovars 1, 2 and 3, respectively. However, some B. suis field isolates identified as biovars 2 or 3 according AMOS-PCR, PCR-RFLP of omp31 and omp2 genes and classical bacteriological methods, resulted also in S1 patterns, limiting the typing usefulness of the method.Entities:
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Year: 2006 PMID： 16530357 DOI： 10.1016/j.vetmic.2006.02.002
Source DB: PubMed Journal: Vet Microbiol ISSN： 0378-1135 Impact factor: 3.293