Literature DB >> 16530187

Gene silencing in mosquito salivary glands by RNAi.

Bertrand Boisson1, Jean Claude Jacques, Valérie Choumet, Estelle Martin, Jiannong Xu, Ken Vernick, Catherine Bourgouin.   

Abstract

Salivary glands are the ultimate site of development in the insect of mosquito born pathogens such as Plasmodium. Mosquito salivary glands also secrete components involved in anti-haemostatic activities and allergic reactions. We investigated the feasibility of RNAi as a tool for functional analysis of genes expressed in Anopheles gambiae salivary glands. We show that specific gene silencing in salivary glands requires the use of large amounts of dsRNA, condition that differs from those for efficient RNAi in other mosquito tissues. Using this protocol, we demonstrated the role of AgApy, which encodes an apyrase, in the probing behaviour of An. gambiae.

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Year:  2006        PMID： 16530187     DOI： 10.1016/j.febslet.2006.02.069

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  47 in total

1.  Knockdown of microplitis mediator odorant receptor involved in the sensitive detection of two chemicals.

Authors:  Ke-Ming Li; Li-Yan Ren; Yong-Jun Zhang; Kong-Ming Wu; Yu-Yuan Guo
Journal:  J Chem Ecol       Date:  2012-03-09       Impact factor: 2.626

2.  Members of the salivary gland surface protein (SGS) family are major immunogenic components of mosquito saliva.

Authors:  Jonas G King; Kenneth D Vernick; Julián F Hillyer
Journal:  J Biol Chem       Date:  2011-09-29       Impact factor: 5.157

3.  Optimization of double-stranded RNAi intrathoracic injection method in Aedes aegypti.

Authors:  Seokyoung Kang; Dongyoung Shin; Mi Young Noh; Jill S Peters; Chelsea T Smartt; Yeon Soo Han; Young S Hong
Journal:  Entomol Res       Date:  2018-07-12       Impact factor: 1.306

Review 4.  Gene expression studies in mosquitoes.

Authors:  Xiao-Guang Chen; Geetika Mathur; Anthony A James
Journal:  Adv Genet       Date:  2008       Impact factor: 1.944

5.  Molecular and functional characterization of Anopheles gambiae inward rectifier potassium (Kir1) channels: a novel role in egg production.

Authors:  Rene Raphemot; Tania Y Estévez-Lao; Matthew F Rouhier; Peter M Piermarini; Jerod S Denton; Julián F Hillyer
Journal:  Insect Biochem Mol Biol       Date:  2014-05-20       Impact factor: 4.714

6.  Dengue virus infection of the Aedes aegypti salivary gland and chemosensory apparatus induces genes that modulate infection and blood-feeding behavior.

Authors:  Shuzhen Sim; José L Ramirez; George Dimopoulos
Journal:  PLoS Pathog       Date:  2012-03-29       Impact factor: 6.823

7.  Anopheles gambiae circumsporozoite protein-binding protein facilitates plasmodium infection of mosquito salivary glands.

Authors:  Jiuling Wang; Yue Zhang; Yang O Zhao; Michelle W M Li; Lili Zhang; Srdjan Dragovic; Nabil M Abraham; Erol Fikrig
Journal:  J Infect Dis       Date:  2013-06-24       Impact factor: 5.226

8.  Collagen-binding protein, Aegyptin, regulates probing time and blood feeding success in the dengue vector mosquito, Aedes aegypti.

Authors:  Andrezza Campos Chagas; José Luis Ramirez; Nijole Jasinskiene; Anthony A James; José M C Ribeiro; Osvaldo Marinotti; Eric Calvo
Journal:  Proc Natl Acad Sci U S A       Date:  2014-04-28       Impact factor: 11.205

9.  The Anopheles gambiae salivary protein gSG6: an anopheline-specific protein with a blood-feeding role.

Authors:  Fabrizio Lombardo; Raffaele Ronca; Cinzia Rizzo; Montserrat Mestres-Simòn; Alessandra Lanfrancotti; Chiara Currà; Gabriella Fiorentino; Catherine Bourgouin; Josè M C Ribeiro; Vincenzo Petrarca; Marta Ponzi; Mario Coluzzi; Bruno Arcà
Journal:  Insect Biochem Mol Biol       Date:  2009-05-12       Impact factor: 4.714

10.  Silencing of carbonic anhydrase in an Anopheles gambiae larval cell line, Ag55.

Authors:  Kristin E Smith; Paul J Linser
Journal:  J RNAi Gene Silencing       Date:  2009-06-17
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