| Literature DB >> 16529715 |
Kaiqin Lao1, Nan Lan Xu, Vivian Yeung, Caifu Chen, Kenneth J Livak, Neil A Straus.
Abstract
MicroRNAs are short (approximately 22 nucleotides), non-coding RNAs that play critical roles in gene regulation and may be used as rapid precise diagnostic indicators of early stages of cancer. The small size of these RNAs makes detection of multiple microRNA species in very small samples problematic. Here we investigate the parameters associated with multiplexing RT-PCR to obtain relative abundance profiles of multiple microRNAs in small sample sizes down to the amount of RNA found in a single cell.Entities:
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Year: 2006 PMID： 16529715 DOI： 10.1016/j.bbrc.2006.02.106
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575