Literature DB >> 16522854

Combination primer polymerase chain reaction for multi-site mutagenesis of close proximity sites.

Pia Hønnerup Jensen1, Dietmar Weilguny.   

Abstract

We describe a rapid and efficient polymerase chain reaction procedure for multi-site-directed mutagenesis for cases in which the sites to be mutated are in close proximity. The combination primer polymer chain reaction method is based on a multi-site directed mutagenesis protocol together with a splicing by overlapping extension polymerase chain reaction protocol. several different combinations of multiple mutations were successfully performed with this method and are reported in this study.

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Year:  2005        PMID： 16522854      PMCID： PMC2291755     

Source DB:  PubMed          Journal:  J Biomol Tech        ISSN： 1524-0215


  9 in total

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Journal:  Biotechniques       Date:  2000-02       Impact factor: 1.993

2.  Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange Site-Directed Mutagenesis.

Authors:  W Wang; B A Malcolm
Journal:  Biotechniques       Date:  1999-04       Impact factor: 1.993

3.  Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis.

Authors:  A Sawano; A Miyawaki
Journal:  Nucleic Acids Res       Date:  2000-08-15       Impact factor: 16.971

4.  Multiple site-directed mutagenesis of more than 10 sites simultaneously and in a single round.

Authors:  Andreas Seyfang; Jean Huaqian Jin
Journal:  Anal Biochem       Date:  2004-01-15       Impact factor: 3.365

5.  An efficient one-step site-directed and site-saturation mutagenesis protocol.

Authors:  Lei Zheng; Ulrich Baumann; Jean-Louis Reymond
Journal:  Nucleic Acids Res       Date:  2004-08-10       Impact factor: 16.971

6.  A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.

Authors:  R Higuchi; B Krummel; R K Saiki
Journal:  Nucleic Acids Res       Date:  1988-08-11       Impact factor: 16.971

7.  The "megaprimer" method of site-directed mutagenesis.

Authors:  G Sarkar; S S Sommer
Journal:  Biotechniques       Date:  1990-04       Impact factor: 1.993

8.  Site-directed mutagenesis by overlap extension using the polymerase chain reaction.

Authors:  S N Ho; H D Hunt; R M Horton; J K Pullen; L R Pease
Journal:  Gene       Date:  1989-04-15       Impact factor: 3.688

9.  An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes.

Authors:  R D Kirsch; E Joly
Journal:  Nucleic Acids Res       Date:  1998-04-01       Impact factor: 16.971
  9 in total
  3 in total

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Journal:  Cell       Date:  2009-10-30       Impact factor: 41.582

2.  Generation of human PTH1R construct with FLAG epitope located internally: comparison of two-fragment assembly by using PCR overlap extension or ligase.

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Journal:  J Biomol Tech       Date:  2009-09

3.  An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol.

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  3 in total

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