| Literature DB >> 16522787 |
S E Burastero1, C Paolucci, D Breda, G Monasterolo, R E Rossi, L Vangelista.
Abstract
The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-FcepsilonRI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.Entities:
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Year: 2006 PMID: 16522787 PMCID: PMC1391964 DOI: 10.1128/CVI.13.3.420-422.2006
Source DB: PubMed Journal: Clin Vaccine Immunol ISSN: 1556-679X