Literature DB >> 1651671

Assay of neomycin phosphotransferase activity in cell extracts.

N Ramesh1, W R Osborne.   

Abstract

A simple method for the measurement of neomycin phosphotransferase (NPT) activity in crude extracts of eukaryotic cells is described. This method is based on the elimination of interfering phosphorylated proteins by using phenol-chloroform extraction. This solution phase assay allows the detection of greater than or equal to 0.01 ng of NPT in the crude cell extract. Rapid screening of a large number of cell cultures generated in gene-transfer experiments, using NPT as a selective marker, is made possible by this simple technique. Further, the promoter strength of vector constructs used in gene therapy may also be estimated by this procedure.

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Year:  1991        PMID： 1651671     DOI： 10.1016/0003-2697(91)90028-r

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  10 in total

1.  Characterization of an internal ribosomal entry segment in the 5' leader of murine leukemia virus env RNA.

Authors:  C Deffaud; J L Darlix
Journal:  J Virol       Date:  2000-01       Impact factor: 5.103

2.  Rous sarcoma virus translation revisited: characterization of an internal ribosome entry segment in the 5' leader of the genomic RNA.

Authors:  C Deffaud; J L Darlix
Journal:  J Virol       Date:  2000-12       Impact factor: 5.103

3.  Characterization of two distinct RNA domains that regulate translation of the Drosophila gypsy retroelement.

Authors:  Corinne Ronfort; Sylvain De Breyne; Virginie Sandrin; Jean-Luc Darlix; Théophile Ohlmann
Journal:  RNA       Date:  2004-03       Impact factor: 4.942

4.  Internal initiation of translation in retroviral vectors carrying picornavirus 5' nontranslated regions.

Authors:  M A Adam; N Ramesh; A D Miller; W R Osborne
Journal:  J Virol       Date:  1991-09       Impact factor: 5.103

5.  High-titer bicistronic retroviral vectors employing foot-and-mouth disease virus internal ribosome entry site.

Authors:  N Ramesh; S T Kim; M Q Wei; M Khalighi; W R Osborne
Journal:  Nucleic Acids Res       Date:  1996-07-15       Impact factor: 16.971

6.  A set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparum.

Authors:  C B Mamoun; I Y Gluzman; S Goyard; S M Beverley; D E Goldberg
Journal:  Proc Natl Acad Sci U S A       Date:  1999-07-20       Impact factor: 11.205

7.  Identification of an internal ribosome entry segment in the 5' region of the mouse VL30 retrotransposon and its use in the development of retroviral vectors.

Authors:  M López-Lastra; S Ulrici; C Gabus; J L Darlix
Journal:  J Virol       Date:  1999-10       Impact factor: 5.103

8.  Kanamycin resistance as a selectable marker for plastid transformation in tobacco.

Authors:  H Carrer; T N Hockenberry; Z Svab; P Maliga
Journal:  Mol Gen Genet       Date:  1993-10

9.  A dormant internal ribosome entry site controls translation of feline immunodeficiency virus.

Authors:  Valentina Camerini; Didier Decimo; Laurent Balvay; Mauro Pistello; Mauro Bendinelli; Jean-Luc Darlix; Théophile Ohlmann
Journal:  J Virol       Date:  2008-01-30       Impact factor: 5.103

10.  Transgenic Leishmania model for delta-aminolevulinate-inducible monospecific uroporphyria: cytolytic phototoxicity initiated by singlet oxygen-mediated inactivation of proteins and its ablation by endosomal mobilization of cytosolic uroporphyrin.

Authors:  Sujoy Dutta; Bala Krishna Kolli; Aihua Tang; Shigeru Sassa; Kwang-Poo Chang
Journal:  Eukaryot Cell       Date:  2008-05-16
  10 in total

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