Input-output relations of the feedback synapse between horizontal cells and cones in the tiger salamander retina.

1. The input-output relation of the feedback synapse between horizontal cells (HCs) and cones was studied by simultaneously recording the light responses of the HCs and of cones the outer segments of which were truncated off. 2. The postsynaptic light response of the truncated cone was depolarizing and free of direct influence of photocurrents. These postsynaptic light responses were graded and sustained; their waveform resembled that of the HC light responses. 3. Input-output relation of the HC-cone feedback synapse was obtained by plotting the simultaneous voltage points of the HC and truncated cone light responses. At the resting potential of the cone (-40 mV), the voltage gain of the feedback synapse was about -0.33 when VHC = -20 mV and it was about -0.03 when VHC = -60 mV. 4. At more hyperpolarized cone voltages, the feedback signals in cones became smaller, and they reversed at about -67 mV. The voltage gain of the feedback synapse at VHC = -20 mV was about -0.23, -0.18, -0.07, and +0.2 when Vcone = -44.5, -52.5, -65, and -77.5 mV, respectively. 5. Light hyperpolarized the HC, which resulted in a conductance change (delta Gs) in cones. The cone conductance decreased progressively as the HCs were increasingly hyperpolarized, and delta Gs reached a maximum value of 0.93 nS when the HCs were hyperpolarized from -20 to -52 mV. 6. The peak light responses of intact cones were plotted against the peak HC light responses. This gives the relationship between the pre- and postsynaptic voltages of the cone-HC (forward) and HC-cone (feedback) synapses at any given light intensity. Combining this relationship with the input-output relations obtained at various voltages of the truncated cones allows the input-output relation of the feedback synapse for light-evoked signals to be obtained. 7. The input-output relation of the feedback synapse for light-evoked signals was bell-shaped, because the feedback light responses were controlled by two opposing factors: as the light became brighter, the postsynaptic conductance change increased, but the driving force decreased. 8. For light-evoked signals, the slope gain (slope of the input-output relation) of the HC-cone feedback synapse was negative (varied from -0.33 to 0) when VHC lay between -20 and -52 mV; and it was positive (0 to +0.8) when VHC lay between -52 and -72 mV. 9.(ABSTRACT TRUNCATED AT 400 WORDS)