Literature DB >> 16511297

Crystallization and preliminary crystallographic analysis of selenomethionine-labelled progesterone 5beta-reductase from Digitalis lanata Ehrh.

Claudia Egerer-Sieber1, Vanessa Herl, Frieder Müller-Uri, Wolfgang Kreis, Yves A Muller.   

Abstract

Progesterone 5beta-reductase (5beta-POR) catalyzes the reduction of progesterone to 5beta-pregnane-3,20-dione and is the first stereospecific enzyme in the putative biosynthetic pathway of Digitalis cardenolides. Selenomethionine-derivatized 5beta-POR from D. lanata was successfully overproduced and crystallized. The crystals belong to space group P4(3)2(1)2, with unit-cell parameters a = 71.73, c = 186.64 A. A MAD data set collected at 2.7 A resolution allowed the identification of six out of eight possible Se-atom positions. A first inspection of the MAD-phased electron-density map shows that 5beta-POR is a Rossmann-type reductase and the quality of the map is such that it is anticipated that a complete atomic model of 5beta-POR will readily be built.

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Year:  2006        PMID： 16511297      PMCID： PMC2197193          DOI： 10.1107/S1744309106001916

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  10 in total

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Journal:  Nucleic Acids Res       Date:  2004-01-01       Impact factor: 16.971

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Journal:  J Mol Biol       Date:  1976-07-25       Impact factor: 5.469

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Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

5.  A stereospecific enzyme of the putative biosynthetic pathway of cardenolides. Characterization of a progesterone 5 beta-reductase from leaves of Digitalis purpurea L.

Authors:  D E Gärtner; S Wendroth; H U Seitz
Journal:  FEBS Lett       Date:  1990-10-01       Impact factor: 4.124

6.  Molecular cloning and heterologous expression of progesterone 5beta-reductase from Digitalis lanata Ehrh.

Authors:  Vanessa Herl; Gabriele Fischer; Frieder Müller-Uri; Wolfgang Kreis
Journal:  Phytochemistry       Date:  2005-12-28       Impact factor: 4.072

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Authors:  T C Terwilliger; J Berendzen
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-04

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Journal:  Eur J Biochem       Date:  1995-06-01

9.  Purification, characterization and partial peptide microsequencing of progesterone 5 beta-reductase from shoot cultures of Digitalis purpurea.

Authors:  D E Gärtner; W Keilholz; H U Seitz
Journal:  Eur J Biochem       Date:  1994-11-01

10.  Maximum-likelihood density modification.

Authors:  T C Terwilliger
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2000-08
  10 in total

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