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Literature DB >> 16511274

Crystallization and preliminary X-ray diffraction studies of two thermostable alpha-galactosidases from glycoside hydrolase family 36.

M Foucault¹, H Watzlawick, R Mattes, R Haser, P Gouet.

Abstract

alpha-Galactosidases from thermophilic organisms have gained interest owing to their applications in the sugar industry. The alpha-galactosidases AgaA, AgaB and AgaA A355E mutant from Geobacillus stearothermophilus have been overexpressed in Escherichia coli. Crystals of AgaB and AgaA A355E have been obtained by the vapour-diffusion method and synchrotron data have been collected to 2.0 and 2.8 A resolution, respectively. Crystals of AgaB belong to space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 87.5, b = 113.3, c = 161.6 A. Crystals of AgaA A355E belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 150.1, c = 233.2 A.

Entities: Chemical Mutation Species

Mesh：

Substances：

Year: 2006 PMID： 16511274 PMCID： PMC2150962 DOI： 10.1107/S1744309105042582

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

18 in total

1. Classification of glycoside hydrolases and glycosyltransferases from hyperthermophiles.

Authors: B Henrissat; P M Coutinho
Journal: Methods Enzymol Date: 2001 Impact factor: 1.600

2. Life with no sugars?

Authors: P M Coutinho; B Henrissat
Journal: J Mol Microbiol Biotechnol Date: 1999-11

3. Implementation of molecular replacement in AMoRe.

Authors: J Navaza
Journal: Acta Crystallogr D Biol Crystallogr Date: 2001-09-21

4. Crystal structure of alpha-galactosidase from Trichoderma reesei and its complex with galactose: implications for catalytic mechanism.

Authors: A M Golubev; R A P Nagem; J R Brandão Neto; K N Neustroev; E V Eneyskaya; A A Kulminskaya; K A Shabalin; A N Savel'ev; I Polikarpov
Journal: J Mol Biol Date: 2004-05-28 Impact factor: 5.469

2. The molecular mechanism of thermostable α-galactosidases AgaA and AgaB explained by x-ray crystallography and mutational studies.

Authors: Romain Merceron; Marine Foucault; Richard Haser; Ralf Mattes; Hildegard Watzlawick; Patrice Gouet
Journal: J Biol Chem Date: 2012-09-25 Impact factor: 5.157

2 in total

Crystallization and preliminary X-ray diffraction studies of two thermostable alpha-galactosidases from glycoside hydrolase family 36.

1. Classification of glycoside hydrolases and glycosyltransferases from hyperthermophiles.

2. Life with no sugars?

3. Implementation of molecular replacement in AMoRe.

4. Crystal structure of alpha-galactosidase from Trichoderma reesei and its complex with galactose: implications for catalytic mechanism.

5. Purification, characterization, and cDNA cloning of a novel alpha-galactosidase from Mortierella vinacea.

6. Substrate specificities of Penicillium simplicissimum alpha-galactosidases.

7. Solvent content of protein crystals.

8. The 1.9 A structure of alpha-N-acetylgalactosaminidase: molecular basis of glycosidase deficiency diseases.

9. The molecular defect leading to Fabry disease: structure of human alpha-galactosidase.

10. Crystallization and preliminary X-ray crystallographic studies of alpha-galactosidase I from Mortierella vinacea.

1. The melREDCA Operon Encodes a Utilization System for the Raffinose Family of Oligosaccharides in Bacillus subtilis.

2. The molecular mechanism of thermostable α-galactosidases AgaA and AgaB explained by x-ray crystallography and mutational studies.