Literature DB >> 16511239

Preparation, crystallization and preliminary X-ray crystallographic studies of diadenosine tetraphosphate hydrolase from Shigella flexneri 2a.

Wenxin Hu1, Qihai Wang, Ruchang Bi.   

Abstract

Diadenosine tetraphosphate (Ap4A) hydrolase (EC 3.6.1.41) hydrolyzes Ap4A symmetrically in prokaryotes. It plays a potential role in organisms by regulating the concentration of Ap4A in vivo. To date, no three-dimensional structures of proteins with significant sequence homology to this protein have been determined. The 31.3 kDa Ap4A hydrolase from Shigella flexneri 2a has been cloned, expressed and purified using an Escherichia coli expression system. Crystals of Ap4A hydrolase have been obtained by the hanging-drop technique at 291 K using PEG 550 MME as precipitant. Ap4A hydrolase crystals diffract X-rays to 3.26 A and belong to space group P2(1), with unit-cell parameters a = 118.9, b = 54.6, c = 128.5 A, beta = 95.7 degrees.

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Year:  2005        PMID： 16511239      PMCID： PMC1978153          DOI： 10.1107/S174430910503722X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  9 in total

1.  The timing of cell division: Ap4A as a signal.

Authors:  A Nishimura
Journal:  Trends Biochem Sci       Date:  1998-05       Impact factor: 13.807

2.  Processing of X-ray diffraction data collected in oscillation mode.

Authors:  Z Otwinowski; W Minor
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600

3.  Diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) controls the timing of cell division in Escherichia coli.

Authors:  A Nishimura; S Moriya; H Ukai; K Nagai; M Wachi; Y Yamada
Journal:  Genes Cells       Date:  1997-06       Impact factor: 1.891

Review 4.  Specific and nonspecific enzymes involved in the catabolism of mononucleoside and dinucleoside polyphosphates.

Authors:  A Guranowski
Journal:  Pharmacol Ther       Date:  2000 Aug-Sep       Impact factor: 12.310

5.  The crystal structure of diadenosine tetraphosphate hydrolase from Caenorhabditis elegans in free and binary complex forms.

Authors:  Scott Bailey; Svetlana E Sedelnikova; G Michael Blackburn; Hend M Abdelghany; Patrick J Baker; Alexander G McLennan; John B Rafferty
Journal:  Structure       Date:  2002-04       Impact factor: 5.006

6.  The structure of Ap(4)A hydrolase complexed with ATP-MgF(x) reveals the basis of substrate binding.

Authors:  Jamie I Fletcher; James D Swarbrick; Danuta Maksel; Kenwyn R Gayler; Paul R Gooley
Journal:  Structure       Date:  2002-02       Impact factor: 5.006

7.  AppppA, heat-shock stress, and cell oxidation.

Authors:  P C Lee; B R Bochner; B N Ames
Journal:  Proc Natl Acad Sci U S A       Date:  1983-12       Impact factor: 11.205

8.  Regiospecificity of the hydrolysis of diadenosine polyphosphates catalyzed by three specific pyrophosphohydrolases.

Authors:  A Guranowski; P Brown; P A Ashton; G M Blackburn
Journal:  Biochemistry       Date:  1994-01-11       Impact factor: 3.162

9.  Conservation analysis and structure prediction of the protein serine/threonine phosphatases. Sequence similarity with diadenosine tetraphosphatase from Escherichia coli suggests homology to the protein phosphatases.

Authors:  G J Barton; P T Cohen; D Barford
Journal:  Eur J Biochem       Date:  1994-02-15
  9 in total

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