Literature DB >> 16511225

Crystallization and X-ray diffraction properties of Baeyer-Villiger monooxygenase MtmOIV from the mithramycin biosynthetic pathway in Streptomyces argillaceus.

Chenchen Wang1, Miranda Gibson, Jurgen Rohr, Marcos A Oliveira.   

Abstract

The Baeyer-Villiger monooxygenase MtmOIV from Streptomyces argillaceus is a 56 kDa FAD-dependent and NADPH-dependent enzyme that is responsible for the key frame-modifying step in the biosynthesis of the natural product mithramycin. Crystals of MtmOIV were flash-cooled and diffracted to 2.69 A resolution using synchrotron radiation on beamline SER-CAT 22-ID at the Advanced Photon Source. Crystals of MtmOIV are monoclinic and light-scattering data reveal that the enzyme forms dimers in solution. The rotation function suggests the presence of two dimers in the asymmetric unit. L-Selenomethionine-incorporated MtmOIV has been obtained. Structural solution combining molecular-replacement phases and anomalous phases from selenium is in progress.

Entities:  

Mesh：

Substances：

Year:  2005        PMID： 16511225      PMCID： PMC1978135          DOI： 10.1107/S1744309105033221

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  15 in total

1.  Purification and characterization of a monooxygenase involved in the biosynthetic pathway of the antitumor drug mithramycin.

Authors:  David Rodríguez; Luis M Quirós; Alfredo F Braña; José A Salas
Journal:  J Bacteriol       Date:  2003-07       Impact factor: 3.490

Review 2.  Towards large-scale synthetic applications of Baeyer-Villiger monooxygenases.

Authors:  Veronique Alphand; Giacomo Carrea; Roland Wohlgemuth; Roland Furstoss; John M Woodley
Journal:  Trends Biotechnol       Date:  2003-07       Impact factor: 19.536

3.  Elucidation of the glycosylation sequence of mithramycin biosynthesis: isolation of 3A-deolivosylpremithramycin B and its conversion to premithramycin B by glycosyltransferase MtmGII.

Authors:  Mohammad Nur-e-Alam; Carmen Méndez; José A Salas; Jürgen Rohr
Journal:  Chembiochem       Date:  2005-04       Impact factor: 3.164

4.  Chromomycin dimer-DNA oligomer complexes. Sequence selectivity and divalent cation specificity.

Authors:  X L Gao; D J Patel
Journal:  Biochemistry       Date:  1990-12-11       Impact factor: 3.162

Review 5.  Sequence-structure analysis of FAD-containing proteins.

Authors:  O Dym; D Eisenberg
Journal:  Protein Sci       Date:  2001-09       Impact factor: 6.725

6.  Ketopremithramycins and ketomithramycins, four new aureolic acid-type compounds obtained upon inactivation of two genes involved in the biosynthesis of the deoxysugar moieties of the antitumor drug mithramycin by Streptomyces argillaceus, reveal novel insights into post-PKS tailoring steps of the mithramycin biosynthetic pathway.

Authors:  Lily L Remsing; Jose Garcia-Bernardo; Ana Gonzalez; Eva Künzel; Uwe Rix; Alfredo F Braña; Daniel W Bearden; Carmen Méndez; Jose A Salas; Jürgen Rohr
Journal:  J Am Chem Soc       Date:  2002-02-27       Impact factor: 15.419

7.  The first fluorogenic assay for detecting a Baeyer-Villigerase activity in microbial cells.

Authors:  María C Gutiérrez; Arthur Sleegers; Helen D Simpson; Véronique Alphand; Roland Furstoss
Journal:  Org Biomol Chem       Date:  2003-10-21       Impact factor: 3.876

8.  Mithramycin SK, a novel antitumor drug with improved therapeutic index, mithramycin SA, and demycarosyl-mithramycin SK: three new products generated in the mithramycin producer Streptomyces argillaceus through combinatorial biosynthesis.

Authors:  Lily L Remsing; Ana M González; Mohammad Nur-e-Alam; M José Fernández-Lozano; Alfredo F Braña; Uwe Rix; Marcos A Oliveira; Carmen Méndez; José A Salas; Jürgen Rohr
Journal:  J Am Chem Soc       Date:  2003-05-14       Impact factor: 15.419

9.  Crystal structure of a Baeyer-Villiger monooxygenase.

Authors:  Enrico Malito; Andrea Alfieri; Marco W Fraaije; Andrea Mattevi
Journal:  Proc Natl Acad Sci U S A       Date:  2004-08-24       Impact factor: 11.205

10.  Identifying determinants of NADPH specificity in Baeyer-Villiger monooxygenases.

Authors:  Nanne M Kamerbeek; Marco W Fraaije; Dick B Janssen
Journal:  Eur J Biochem       Date:  2004-06
View more
  2 in total

1.  Molecular insight into substrate recognition and catalysis of Baeyer-Villiger monooxygenase MtmOIV, the key frame-modifying enzyme in the biosynthesis of anticancer agent mithramycin.

Authors:  Mary A Bosserman; Theresa Downey; Nicholas Noinaj; Susan K Buchanan; Jürgen Rohr
Journal:  ACS Chem Biol       Date:  2013-09-13       Impact factor: 5.100

2.  Crystal structure of Baeyer-Villiger monooxygenase MtmOIV, the key enzyme of the mithramycin biosynthetic pathway .

Authors:  Miranda P Beam; Mary A Bosserman; Nicholas Noinaj; Marie Wehenkel; Jürgen Rohr
Journal:  Biochemistry       Date:  2009-06-02       Impact factor: 3.162
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.