Literature DB >> 16511192

Crystallization and preliminary X-ray crystallographic analysis of Sulfolobus solfataricus thioredoxin reductase.

Alessia Ruggiero1, Maria Rosaria Ruocco, Pasquale Grimaldi, Paolo Arcari, Mariorosario Masullo, Adriana Zagari, Luigi Vitagliano.   

Abstract

A thermostable thioredoxin reductase isolated from Sulfolobus solfataricus (SsTrxR) has been successfully crystallized in the absence and in the presence of NADP. Two different crystal forms have been obtained. Crystals of the form that yields higher resolution data (1.8 A) belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 76.77, b = 120.68, c = 126.85 A. The structure of the enzyme has been solved by MAD methods using the anomalous signal from the Se atoms of selenomethionine-labelled SsTrxR.

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Year:  2005        PMID： 16511192      PMCID： PMC1991316          DOI： 10.1107/S1744309105027995

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  18 in total

1.  Automated protein model building combined with iterative structure refinement.

Authors:  A Perrakis; R Morris; V S Lamzin
Journal:  Nat Struct Biol       Date:  1999-05

2.  Identification and characterization of thioredoxin and thioredoxin reductase from Aeropyrum pernix K1.

Authors:  Sung-Jong Jeon; Kazuhiko Ishikawa
Journal:  Eur J Biochem       Date:  2002-11

Review 3.  The diversity and evolution of thioredoxin reductase: new perspectives.

Authors:  Robert P Hirt; Sylke Müller; T Martin Embley; Graham H Coombs
Journal:  Trends Parasitol       Date:  2002-07

4.  Crystal structure of reduced thioredoxin reductase from Escherichia coli: structural flexibility in the isoalloxazine ring of the flavin adenine dinucleotide cofactor.

Authors:  B W Lennon; C H Williams; M L Ludwig
Journal:  Protein Sci       Date:  1999-11       Impact factor: 6.725

5.  A 35 kDa NAD(P)H oxidase previously isolated from the archaeon Sulfolobus solfataricus is instead a thioredoxin reductase.

Authors:  M R Ruocco; A Ruggiero; L Masullo; P Arcari; M Masullo
Journal:  Biochimie       Date:  2004-12       Impact factor: 4.079

Review 6.  Physiological functions of thioredoxin and thioredoxin reductase.

Authors:  E S Arnér; A Holmgren
Journal:  Eur J Biochem       Date:  2000-10

7.  Twists in catalysis: alternating conformations of Escherichia coli thioredoxin reductase.

Authors:  B W Lennon; C H Williams; M L Ludwig
Journal:  Science       Date:  2000-08-18       Impact factor: 47.728

8.  Automated MAD and MIR structure solution.

Authors:  T C Terwilliger; J Berendzen
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-04

9.  Expression, purification, crystallization and preliminary X-ray crystallographic studies of Mycobacterium tuberculosis thioredoxin reductase.

Authors:  Mohd Akif; Radha Chauhan; Shekhar C Mande
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-03-23

10.  A hyperthermostable novel protein-disulfide oxidoreductase is reduced by thioredoxin reductase from hyperthermophilic archaeon Pyrococcus horikoshii.

Authors:  Yasuhiro Kashima; Kazuhiko Ishikawa
Journal:  Arch Biochem Biophys       Date:  2003-10-15       Impact factor: 4.013
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  2 in total

1.  Crystallization and preliminary X-ray crystallographic analysis of two dimeric hyperthermostable thioredoxins isolated from Sulfolobus solfataricus.

Authors:  Alessia Ruggiero; Maria Angela Lanzotti; Maria Rosaria Ruocco; Pasquale Grimaldi; Daniela Marasco; Paolo Arcari; Mariorosario Masullo; Adriana Zagari; Luigi Vitagliano
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-05-22

2.  Characterisation of the components of the thioredoxin system in the archaeon Sulfolobus solfataricus.

Authors:  Pasquale Grimaldi; Maria Rosaria Ruocco; Maria Angela Lanzotti; Alessia Ruggiero; Immacolata Ruggiero; Paolo Arcari; Luigi Vitagliano; Mariorosario Masullo
Journal:  Extremophiles       Date:  2008-04-17       Impact factor: 2.395
  2 in total

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