Literature DB >> 16511169

Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans.

Catherine Tricot1, Sebastien van Aelst, Ruddy Wattiez, Max Mergeay, Victor Stalon, Johan Wouters.   

Abstract

CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 A, and diffract to 2.2 A resolution using synchrotron radiation.

Entities:  

Mesh：

Substances：

Year:  2005        PMID： 16511169      PMCID： PMC1978110          DOI： 10.1107/S174430910502316X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  10 in total

1.  Genetic and physical maps of the Alcaligenes eutrophus CH34 megaplasmid pMOL28 and its derivative pMOL50 obtained after temperature-induced mutagenesis and mortality.

Authors:  S Taghavi; M Mergeay; D van der Lelie
Journal:  Plasmid       Date:  1997       Impact factor: 3.466

2.  Processing of X-ray diffraction data collected in oscillation mode.

Authors:  Z Otwinowski; W Minor
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600

3.  [29] Preparation of selenomethionyl proteins for phase determination.

Authors:  Sylvie Doublié
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600

4.  Classification of metal-resistant bacteria from industrial biotopes as Ralstonia campinensis sp. nov., Ralstonia metallidurans sp. nov. and Ralstonia basilensis Steinle et al. 1998 emend.

Authors:  J Goris; P De Vos; T Coenye; B Hoste; D Janssens; H Brim; L Diels; M Mergeay; K Kersters; P Vandamme
Journal:  Int J Syst Evol Microbiol       Date:  2001-09       Impact factor: 2.747

5.  Extrachromosomal inheritance controlling resistance to cadmium, cobalt, copper and zinc ions: evidence from curing in a Pseudomonas [proceedings].

Authors:  M Mergeay; C Houba; J Gerits
Journal:  Arch Int Physiol Biochim       Date:  1978-05

6.  Rapid procedure for detection and isolation of large and small plasmids.

Authors:  C I Kado; S T Liu
Journal:  J Bacteriol       Date:  1981-03       Impact factor: 3.490

Review 7.  Ralstonia metallidurans, a bacterium specifically adapted to toxic metals: towards a catalogue of metal-responsive genes.

Authors:  Max Mergeay; Sébastien Monchy; Tatiana Vallaeys; Vanessa Auquier; Abderrafi Benotmane; Philippe Bertin; Safiyh Taghavi; John Dunn; Daniel van der Lelie; Ruddy Wattiez
Journal:  FEMS Microbiol Rev       Date:  2003-06       Impact factor: 16.408

8.  Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. nov.

Authors:  Mario Vaneechoutte; Peter Kämpfer; Thierry De Baere; Enevold Falsen; Gerda Verschraegen
Journal:  Int J Syst Evol Microbiol       Date:  2004-03       Impact factor: 2.747

9.  Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals.

Authors:  M Mergeay; D Nies; H G Schlegel; J Gerits; P Charles; F Van Gijsegem
Journal:  J Bacteriol       Date:  1985-04       Impact factor: 3.490

10.  Taxonomy of the genus Cupriavidus: a tale of lost and found.

Authors:  Peter Vandamme; Tom Coenye
Journal:  Int J Syst Evol Microbiol       Date:  2004-11       Impact factor: 2.747
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.