Literature DB >> 16511139

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapB (Rv2773c) from Mycobacterium tuberculosis.

Georgia Kefala1, Robert Janowski, Santosh Panjikar, Christoph Mueller-Dieckmann, Manfred S Weiss.   

Abstract

Dihydrodipicolinate reductase from Mycobacterium tuberculosis (DapB, DHDPR, Rv2773c) has been cloned and heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in three different crystal forms. Preliminary diffraction data analysis suggests the presence of two tetramers in the asymmetric unit of one crystal form and half a tetramer in the other two crystal forms.

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Year:  2005        PMID： 16511139      PMCID： PMC1952461          DOI： 10.1107/S174430910501938X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  10 in total

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Authors:  Craig A Hutton; Timothy J Southwood; Jennifer J Turner
Journal:  Mini Rev Med Chem       Date:  2003-03       Impact factor: 3.862

2.  The CCP4 suite: programs for protein crystallography.

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Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-09-01

3.  Processing of X-ray diffraction data collected in oscillation mode.

Authors:  Z Otwinowski; W Minor
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600

4.  Three-dimensional structure of Escherichia coli dihydrodipicolinate reductase.

Authors:  G Scapin; J S Blanchard; J C Sacchettini
Journal:  Biochemistry       Date:  1995-03-21       Impact factor: 3.162

5.  Nucleotide sequence and expression of the Escherichia coli dapB gene.

Authors:  J Bouvier; C Richaud; F Richaud; J C Patte; P Stragier
Journal:  J Biol Chem       Date:  1984-12-10       Impact factor: 5.157

6.  Reaction mechanism of Escherichia coli dihydrodipicolinate synthase investigated by X-ray crystallography and NMR spectroscopy.

Authors:  S Blickling; C Renner; B Laber; H D Pohlenz; T A Holak; R Huber
Journal:  Biochemistry       Date:  1997-01-07       Impact factor: 3.162

7.  Three-dimensional structure of Escherichia coli dihydrodipicolinate reductase in complex with NADH and the inhibitor 2,6-pyridinedicarboxylate.

Authors:  G Scapin; S G Reddy; R Zheng; J S Blanchard
Journal:  Biochemistry       Date:  1997-12-09       Impact factor: 3.162

8.  Expression, purification, and characterization of Escherichia coli dihydrodipicolinate reductase.

Authors:  S G Reddy; J C Sacchettini; J S Blanchard
Journal:  Biochemistry       Date:  1995-03-21       Impact factor: 3.162

9.  The three-dimensional structures of the Mycobacterium tuberculosis dihydrodipicolinate reductase-NADH-2,6-PDC and -NADPH-2,6-PDC complexes. Structural and mutagenic analysis of relaxed nucleotide specificity.

Authors:  Maurizio Cirilli; Renjian Zheng; Giovanna Scapin; John S Blanchard
Journal:  Biochemistry       Date:  2003-09-16       Impact factor: 3.162

10.  Influence of the codon following the AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli.

Authors:  A C Looman; J Bodlaender; L J Comstock; D Eaton; P Jhurani; H A de Boer; P H van Knippenberg
Journal:  EMBO J       Date:  1987-08       Impact factor: 11.598
  10 in total
  5 in total

1.  The three-dimensional structure of diaminopimelate decarboxylase from Mycobacterium tuberculosis reveals a tetrameric enzyme organisation.

Authors:  Simone Weyand; Georgia Kefala; Dmitri I Svergun; Manfred S Weiss
Journal:  J Struct Funct Genomics       Date:  2009-06-19

2.  Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of tetrahydrodipicolinate-N-succinyltransferase (Rv1201c) from Mycobacterium tuberculosis.

Authors:  Linda Schuldt; Simone Weyand; Georgia Kefala; Manfred S Weiss
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-08-29

3.  Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the regulatory domain of aspartokinase (Rv3709c) from Mycobacterium tuberculosis.

Authors:  Linda Schuldt; Ruth Suchowersky; Katharina Veith; Jochen Mueller-Dieckmann; Manfred S Weiss
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-02-25

4.  Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of succinyl-diaminopimelate desuccinylase (Rv1202, DapE) from Mycobacterium tuberculosis.

Authors:  Linda Reinhard; Jochen Mueller-Dieckmann; Manfred S Weiss
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-08-31

5.  Cloning, Expression, and Purification of Histidine-Tagged Escherichia coli Dihydrodipicolinate Reductase.

Authors:  Yvonne D Trigoso; Russell C Evans; William E Karsten; Lilian Chooback
Journal:  PLoS One       Date:  2016-01-27       Impact factor: 3.240
  5 in total

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