Literature DB >> 16511109

Crystallization and avoiding the problem of hemihedral twinning in crystals of Delta1-pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus.

Eiji Inagaki1, Hitomi Takahashi, Chizu Kuroishi, Tahir H Tahirov.   

Abstract

Delta1-Pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus (TtP5CDh) has been crystallized in a citrate-bound form (TtP5CDh-cit). The crystals diffracted to well beyond 2 A resolution, but exhibited perfect or near-perfect hemihedral twinning. Variation of crystallization conditions resulted in the growth of larger untwinned crystals or crystals with significantly reduced twin content, all with similar unit-cell parameters. The soaking of TtP5CDh-cit crystals in citrate-free solution produced crystals of the apo form (TtP5CDh-apo). The TtP5CDh-apo crystals belong to space group R3, with unit-cell parameters a = b = 102.29, c = 279.28 A, and diffract to 1.08 A. Crystals soaked in solution with NAD+ (TtP5CDh-NAD), NADH (TtP5CDh-NADH) and glutamate (TtP5CDh-Glu) were also prepared and characterized.

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Year:  2005        PMID： 16511109      PMCID： PMC1952327          DOI： 10.1107/S1744309105016118

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


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  3 in total

1.  Structural determinants of oligomerization of δ(1)-pyrroline-5-carboxylate dehydrogenase: identification of a hexamerization hot spot.

Authors:  Min Luo; Ranjan K Singh; John J Tanner
Journal:  J Mol Biol       Date:  2013-06-07       Impact factor: 5.469

2.  New insights into the binding mode of coenzymes: structure of Thermus thermophilus Delta1-pyrroline-5-carboxylate dehydrogenase complexed with NADP+.

Authors:  Eiji Inagaki; Noriyasu Ohshima; Keiko Sakamoto; Nigar D Babayeva; Hiroaki Kato; Shigeyuki Yokoyama; Tahir H Tahirov
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-05-05

3.  Study of ALDH from Thermus thermophilus-Expression, Purification and Characterisation of the Non-Substrate Specific, Thermophilic Enzyme Displaying Both Dehydrogenase and Esterase Activity.

Authors:  Kim Shortall; Edel Durack; Edmond Magner; Tewfik Soulimane
Journal:  Cells       Date:  2021-12-14       Impact factor: 6.600
  3 in total

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