Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Crystallization and preliminary X-ray analysis of methylthioribose-1-phosphate isomerase from Bacillus subtilis.

Literature DB >> 16511105

Crystallization and preliminary X-ray analysis of methylthioribose-1-phosphate isomerase from Bacillus subtilis.

Haruka Tamura¹, Hiroyoshi Matsumura, Tsuyoshi Inoue, Hiroki Ashida, Yohtaro Saito, Akiho Yokota, Yasushi Kai.

Abstract

Methylthioribose-1-phosphate isomerase (MtnA) from Bacillus subtilis, the first enzyme in the downstream section of the methionine-salvage pathway, was crystallized using the sitting-drop vapour-diffusion method. Crystals grew using ammonium sulfate as the precipitant at 293 K. They diffracted to 2.5 A at 100 K using synchrotron radiation and were found to belong to the tetragonal space group P4(1), with unit-cell parameters a = b = 69.2, c = 154.7 A. The asymmetric unit contains two molecules of MtnA, with a VM value of 2.4 A3 Da(-1) and a solvent content of 48%.

Entities: Chemical Species

Mesh：

Substances：

Year: 2005 PMID： 16511105 PMCID： PMC1952323 DOI： 10.1107/S1744309105015757

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

19 in total

Review 1. Mob psychology.

Authors: Stephen C Winans; Bonnie L Bassler
Journal: J Bacteriol Date: 2002-02 Impact factor: 3.490

2. Crystal structure of the regulatory subunit of archaeal initiation factor 2B (aIF2B) from hyperthermophilic archaeon Pyrococcus horikoshii OT3: a proposed structure of the regulatory subcomplex of eukaryotic IF2B.

Authors: Yoshimitsu Kakuta; Maino Tahara; Shigehiro Maetani; Min Yao; Isao Tanaka; Makoto Kimura
Journal: Biochem Biophys Res Commun Date: 2004-07-02 Impact factor: 3.575

3. The CCP4 suite: programs for protein crystallography.

Authors:
Journal: Acta Crystallogr D Biol Crystallogr Date: 1994-09-01

4. 5-Methylthioribose kinase. A new enzyme involved in the formation of methionine from 5-methylthioribose.

Authors: A J Ferro; A Barrett; S K Shapiro
Journal: J Biol Chem Date: 1978-09-10 Impact factor: 5.157

5. Prediction of gene function in methylthioadenosine recycling from regulatory signals.

Authors: Brooke A Murphy; Frank J Grundy; Tina M Henkin
Journal: J Bacteriol Date: 2002-04 Impact factor: 3.490

6. The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria.

Authors: F J Grundy; T M Henkin
Journal: Mol Microbiol Date: 1998-11 Impact factor: 3.501

1. Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis: implications for catalytic mechanism.

Authors: Haruka Tamura; Yohtaro Saito; Hiroki Ashida; Tsuyoshi Inoue; Yasushi Kai; Akiho Yokota; Hiroyoshi Matsumura
Journal: Protein Sci Date: 2008-01 Impact factor: 6.725

1 in total

Crystallization and preliminary X-ray analysis of methylthioribose-1-phosphate isomerase from Bacillus subtilis.

Review 1. Mob psychology.

2. Crystal structure of the regulatory subunit of archaeal initiation factor 2B (aIF2B) from hyperthermophilic archaeon Pyrococcus horikoshii OT3: a proposed structure of the regulatory subcomplex of eukaryotic IF2B.

3. The CCP4 suite: programs for protein crystallography.

4. 5-Methylthioribose kinase. A new enzyme involved in the formation of methionine from 5-methylthioribose.

5. Prediction of gene function in methylthioadenosine recycling from regulatory signals.

6. The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria.

7. A functional link between RuBisCO-like protein of Bacillus and photosynthetic RuBisCO.

8. Methylthioadenosine phosphorylase regulates ornithine decarboxylase by production of downstream metabolites.

9. Crystal structure of yeast Ypr118w, a methylthioribose-1-phosphate isomerase related to regulatory eIF2B subunits.

10. Bacterial variations on the methionine salvage pathway.

1. Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis: implications for catalytic mechanism.