Literature DB >> 16511084

Crystallization and preliminary crystallographic analysis of PimA, an essential mannosyltransferase from Mycobacterium smegmatis.

Marcelo E Guerin1, Alejandro Buschiazzo, Jana Korduláková, Mary Jackson, Pedro M Alzari.   

Abstract

Phosphatidylinositol mannosyltransferase (PimA) is an essential enzyme for mycobacterial growth that catalyses the first mannosylation step in phosphatidyl-myo-inositol mannoside (PIM) biosynthesis. The enzyme belongs to the large GT4 family of glycosyltransferases, for which no structure is currently available. Recombinant purified PimA from Mycobacterium smegmatis has been crystallized in the presence of GDP and myo-inositol. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 37.2, b = 72.4, c = 138.2 A, and diffract to 2.4 A resolution.

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Year:  2005        PMID： 16511084      PMCID： PMC1952298          DOI： 10.1107/S1744309105012364

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  13 in total

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3.  The pimB gene of Mycobacterium tuberculosis encodes a mannosyltransferase involved in lipoarabinomannan biosynthesis.

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4.  Phosphatidylinositol is an essential phospholipid of mycobacteria.

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Journal:  J Biol Chem       Date:  2000-09-29       Impact factor: 5.157

5.  Characterization of a putative alpha-mannosyltransferase involved in phosphatidylinositol trimannoside biosynthesis in Mycobacterium tuberculosis.

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Journal:  Biochem J       Date:  2002-05-01       Impact factor: 3.857

6.  Identification of the required acyltransferase step in the biosynthesis of the phosphatidylinositol mannosides of mycobacterium species.

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Review 8.  Mycobacterial lipoarabinomannan and related lipoglycans: from biogenesis to modulation of the immune response.

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10.  Definition of the first mannosylation step in phosphatidylinositol mannoside synthesis. PimA is essential for growth of mycobacteria.

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  5 in total

1.  New insights into the early steps of phosphatidylinositol mannoside biosynthesis in mycobacteria: PimB' is an essential enzyme of Mycobacterium smegmatis.

Authors:  Marcelo E Guerin; Devinder Kaur; B S Somashekar; Sara Gibbs; Petra Gest; Delphi Chatterjee; Patrick J Brennan; Mary Jackson
Journal:  J Biol Chem       Date:  2009-07-28       Impact factor: 5.157

2.  Tryptophan residues promote membrane association for a plant lipid glycosyltransferase involved in phosphate stress.

Authors:  Changrong Ge; Alexander Georgiev; Anders Öhman; Åke Wieslander; Amélie A Kelly
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3.  Purification and characterization of the acyltransferase involved in biosynthesis of the major mycobacterial cell envelope glycolipid--monoacylated phosphatidylinositol dimannoside.

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4.  Molecular Basis of Membrane Association by the Phosphatidylinositol Mannosyltransferase PimA Enzyme from Mycobacteria.

Authors:  Ane Rodrigo-Unzueta; Mariano A Martínez; Natalia Comino; Pedro M Alzari; Alexandre Chenal; Marcelo E Guerin
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5.  Substrate-induced conformational changes in the essential peripheral membrane-associated mannosyltransferase PimA from mycobacteria: implications for catalysis.

Authors:  Marcelo E Guerin; Francis Schaeffer; Alain Chaffotte; Petra Gest; David Giganti; Jana Korduláková; Mark van der Woerd; Mary Jackson; Pedro M Alzari
Journal:  J Biol Chem       Date:  2009-06-11       Impact factor: 5.157
  5 in total

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