Literature DB >> 16511048

Expression, purification and crystallization of a human protein SH3BGRL at atomic resolution.

Lei Yin1, De-Yu Zhu, Na Yang, Qiu-Hua Huang, Ying Zhang, Da-Cheng Wang.   

Abstract

The protein SH3BGRL, containing both SH3-binding and Homer EVH1-binding motifs, has been crystallized using the hanging-drop vapour-diffusion method. The crystals diffract to 0.88 A resolution and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 28.8886, b = 34.9676, c = 98.0016 A. Preliminary analysis indicates that the asymmetric unit contains one molecule and has a solvent content of about 34%.

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Year:  2005        PMID： 16511048      PMCID： PMC1952435          DOI： 10.1107/S174430910500730X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  10 in total

1.  X chromosome-specific cDNA arrays: identification of genes that escape from X-inactivation and other applications.

Authors:  R Sudbrak; G Wieczorek; U A Nuber; W Mann; R Kirchner; F Erdogan; C J Brown; D Wöhrle; P Sterk; V M Kalscheuer; W Berger; H Lehrach; H H Ropers
Journal:  Hum Mol Genet       Date:  2001-01-01       Impact factor: 6.150

2.  Structure of the Homer EVH1 domain-peptide complex reveals a new twist in polyproline recognition.

Authors:  J Beneken; J C Tu; B Xiao; M Nuriya; J P Yuan; P F Worley; D J Leahy
Journal:  Neuron       Date:  2000-04       Impact factor: 17.173

3.  The CCP4 suite: programs for protein crystallography.

Authors: 
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-09-01

4.  A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family.

Authors:  K Niebuhr; F Ebel; R Frank; M Reinhard; E Domann; U D Carl; U Walter; F B Gertler; J Wehland; T Chakraborty
Journal:  EMBO J       Date:  1997-09-01       Impact factor: 11.598

5.  Two binding orientations for peptides to the Src SH3 domain: development of a general model for SH3-ligand interactions.

Authors:  S Feng; J K Chen; H Yu; J A Simon; S L Schreiber
Journal:  Science       Date:  1994-11-18       Impact factor: 47.728

6.  Identification and characterization of a new human gene encoding a small protein with high homology to the proline-rich region of the SH3BGR gene.

Authors:  A Egeo; M Mazzocco; P Arrigo; J M Vidal-Taboada; R Oliva; B Pirola; S Giglio; A Rasore-Quartino; P Scartezzini
Journal:  Biochem Biophys Res Commun       Date:  1998-06-18       Impact factor: 3.575

Review 7.  Signaling through scaffold, anchoring, and adaptor proteins.

Authors:  T Pawson; J D Scott
Journal:  Science       Date:  1997-12-19       Impact factor: 47.728

Review 8.  Modular binding domains in signal transduction proteins.

Authors:  G B Cohen; R Ren; D Baltimore
Journal:  Cell       Date:  1995-01-27       Impact factor: 41.582

9.  The identification of a novel human homologue of the SH3 binding glutamic acid-rich (SH3BGR) gene establishes a new family of highly conserved small proteins related to Thioredoxin Superfamily.

Authors:  Michela Mazzocco; Massimo Maffei; Aliana Egeo; Alessandro Vergano; Patrizio Arrigo; Raffaella Di Lisi; Fabio Ghiotto; Paolo Scartezzini
Journal:  Gene       Date:  2002-05-29       Impact factor: 3.688

10.  Identification of a protein that binds to the SH3 region of Abl and is similar to Bcr and GAP-rho.

Authors:  P Cicchetti; B J Mayer; G Thiel; D Baltimore
Journal:  Science       Date:  1992-08-07       Impact factor: 47.728
  10 in total

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