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Literature DB >> 16511018

Overproduction, purification, crystallization and preliminary X-ray diffraction studies of the human spliceosomal protein TXNL4B.

Tengchuan Jin¹, Andrew J Howard, Erica A Golemis, Yingtong Wang, Yu-Zhu Zhang.

Abstract

The human gene coding for the spliceosomal protein thioredoxin-like 4B (TXNL4B) was overexpressed in Escherichia coli and the encoded protein was purified and crystallized. Well diffracting single crystals were obtained by the vapor-diffusion method in hanging drops. The crystals belong to the primitive monoclinic space group P2, with unit-cell parameters a = 39.0, b = 63.6, c = 51.0 A, beta = 92.484 degrees,, and diffract to at least 1.50 A. A SeMet derivative of the protein was prepared and crystallized for MAD phasing.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 2005 PMID： 16511018 PMCID： PMC1952293 DOI： 10.1107/S1744309105002861

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

13 in total

1. Identification, characterization and crystal structure analysis of the human spliceosomal U5 snRNP-specific 15 kD protein.

Authors: K Reuter; S Nottrott; P Fabrizio; R Lührmann; R Ficner
Journal: J Mol Biol Date: 1999-11-26 Impact factor: 5.469

2. The evolutionarily conserved Dim1 protein defines a novel branch of the thioredoxin fold superfamily.

Authors: Y Z Zhang; K L Gould; J R Dunbrack RL; H Cheng; H Roder; E A Golemis
Journal: Physiol Genomics Date: 1999-11-11 Impact factor: 3.107

3. Comprehensive proteomic analysis of the human spliceosome.

Authors: Zhaolan Zhou; Lawrence J Licklider; Steven P Gygi; Robin Reed
Journal: Nature Date: 2002-09-12 Impact factor: 49.962

4. DLP, a novel Dim1 family protein implicated in pre-mRNA splicing and cell cycle progression.

Authors: Xiaojing Sun; Hua Zhang; Dan Wang; Dalong Ma; Yan Shen; Yongfeng Shang
Journal: J Biol Chem Date: 2004-05-25 Impact factor: 5.157

5. Processing of X-ray diffraction data collected in oscillation mode.

Authors: Z Otwinowski; W Minor
Journal: Methods Enzymol Date: 1997 Impact factor: 1.600

6. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae.

Authors: P Uetz; L Giot; G Cagney; T A Mansfield; R S Judson; J R Knight; D Lockshon; V Narayan; M Srinivasan; P Pochart; A Qureshi-Emili; Y Li; B Godwin; D Conover; T Kalbfleisch; G Vijayadamodar; M Yang; M Johnston; S Fields; J M Rothberg
Journal: Nature Date: 2000-02-10 Impact factor: 49.962

7. Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin.

Authors: C Oubridge; N Ito; P R Evans; C H Teo; K Nagai
Journal: Nature Date: 1994-12-01 Impact factor: 49.962

8. Evidence that dim1 associates with proteins involved in pre-mRNA splicing, and delineation of residues essential for dim1 interactions with hnRNP F and Npw38/PQBP-1.

Authors: Y Zhang; T Lindblom; A Chang; M Sudol; A E Sluder; E A Golemis
Journal: Gene Date: 2000-10-17 Impact factor: 3.688

Overproduction, purification, crystallization and preliminary X-ray diffraction studies of the human spliceosomal protein TXNL4B.

1. Identification, characterization and crystal structure analysis of the human spliceosomal U5 snRNP-specific 15 kD protein.

2. The evolutionarily conserved Dim1 protein defines a novel branch of the thioredoxin fold superfamily.

3. Comprehensive proteomic analysis of the human spliceosome.

4. DLP, a novel Dim1 family protein implicated in pre-mRNA splicing and cell cycle progression.

5. Processing of X-ray diffraction data collected in oscillation mode.

6. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae.

7. Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin.

8. Evidence that dim1 associates with proteins involved in pre-mRNA splicing, and delineation of residues essential for dim1 interactions with hnRNP F and Npw38/PQBP-1.

9. Structure, stability, and function of hDim1 investigated by NMR, circular dichroism, and mutational analysis.

10. NMR studies of U1 snRNA recognition by the N-terminal RNP domain of the human U1A protein.

1. High-resolution crystal structure of human Dim2/TXNL4B.

2. Construction of two recombination yeast two-hybrid vectors by in vitro recombination.