Literature DB >> 16511009

Crystallization and preliminary crystallographic analysis of the cellulose biosynthesis-related protein CMCax from Acetobacter xylinum.

Shin Kawano1, Yoshiaki Yasutake, Kenji Tajima, Yasuharu Satoh, Min Yao, Isao Tanaka, Masanobu Munekata.   

Abstract

The cellulose biosynthesis-related protein CMCax from Acetobacter xylinum was overexpressed in Escherichia coli, purified and crystallized. Single crystals of selenomethionine (SeMet) substituted CMCax were obtained by the hanging-drop vapour-diffusion method at 293 K, primarily using polyethylene glycol 4000 as a precipitant. The crystals belong to the primitive hexagonal space group P6(1) or P6(5), with unit-cell parameters a = b = 89.1, c = 94.2 A. The predicted Matthews coefficient (VM) value is 3.0 A3 Da(-1) for one CMCax monomer in the asymmetric unit. A single-wavelength anomalous dispersion (SAD) data set was collected to a resolution of 2.3 A using synchrotron radiation.

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Year:  2005        PMID： 16511009      PMCID： PMC1952249          DOI： 10.1107/S174430910500206X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  14 in total

1.  The structure of a cold-adapted family 8 xylanase at 1.3 A resolution. Structural adaptations to cold and investgation of the active site.

Authors:  Filip Van Petegem; Tony Collins; Marie-Alice Meuwis; Charles Gerday; Georges Feller; Jozef Van Beeumen
Journal:  J Biol Chem       Date:  2002-12-09       Impact factor: 5.157

2.  Cloning of cellulose synthesis related genes from Acetobacter xylinum ATCC23769 and ATCC53582: comparison of cellulose synthetic ability between strains.

Authors:  Shin Kawano; Kenji Tajima; Yukako Uemori; Hitomi Yamashita; Tomoki Erata; Masanobu Munekata; Mitsuo Takai
Journal:  DNA Res       Date:  2002-10-31       Impact factor: 4.458

3.  Processing of X-ray diffraction data collected in oscillation mode.

Authors:  Z Otwinowski; W Minor
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600

4.  Atomic (0.94 A) resolution structure of an inverting glycosidase in complex with substrate.

Authors:  Diego M A Guérin; Marie-Bernard Lascombe; Marcelo Costabel; Hélène Souchon; Victor Lamzin; Pierre Béguin; Pedro M Alzari
Journal:  J Mol Biol       Date:  2002-03-08       Impact factor: 5.469

5.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

6.  Effects of endogenous endo-beta-1,4-glucanase on cellulose biosynthesis in Acetobacter xylinum ATCC23769.

Authors:  Shin Kawano; Kenji Tajima; Hiroyuki Kono; Tomoki Erata; Masanobu Munekata; Mitsuo Takai
Journal:  J Biosci Bioeng       Date:  2002       Impact factor: 2.894

7.  Genetic organization of the cellulose synthase operon in Acetobacter xylinum.

Authors:  H C Wong; A L Fear; R D Calhoon; G H Eichinger; R Mayer; D Amikam; M Benziman; D H Gelfand; J H Meade; A W Emerick
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

8.  A new gene required for cellulose production and a gene encoding cellulolytic activity in Acetobacter xylinum are colocalized with the bcs operon.

Authors:  R Standal; T G Iversen; D H Coucheron; E Fjaervik; J M Blatny; S Valla
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

9.  Characterization of genes in the cellulose-synthesizing operon (acs operon) of Acetobacter xylinum: implications for cellulose crystallization.

Authors:  I M Saxena; K Kudlicka; K Okuda; R M Brown
Journal:  J Bacteriol       Date:  1994-09       Impact factor: 3.490

10.  Crystal structure of family GH-8 chitosanase with subclass II specificity from Bacillus sp. K17.

Authors:  Wataru Adachi; Yuri Sakihama; Shinji Shimizu; Tomoko Sunami; Tetsuya Fukazawa; Mamie Suzuki; Rie Yatsunami; Satoshi Nakamura; Akio Takénaka
Journal:  J Mol Biol       Date:  2004-10-22       Impact factor: 5.469
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