Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 The purification and crystallization of mDia1 in complex with RhoC.

Literature DB >> 16511001

The purification and crystallization of mDia1 in complex with RhoC.

Rolf Rose¹, Alfred Wittinghofer, Michael Weyand.

Abstract

An N-terminal construct of mouse mDia1 was recombinantly expressed in Escherichia coli, purified and crystallized in complex with truncated human RhoC using the hanging-drop vapour-diffusion method. Crystals were obtained using PEG 2K MME and MgSO4 as a precipitating agent and belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 148.4, b = 85.2, c = 123.2 A. Complete native and SeMet-derivative data sets were collected at 100 K to 3.0 and 3.4 A resolution, respectively, using synchrotron radiation.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 2005 PMID： 16511001 PMCID： PMC1952246 DOI： 10.1107/S1744309105001065

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

10 in total

1. Identification of a carboxyl-terminal diaphanous-related formin homology protein autoregulatory domain.

Authors: A S Alberts
Journal: J Biol Chem Date: 2000-10-16 Impact factor: 5.157

Review 2. Rho GTPases in cell biology.

Authors: Sandrine Etienne-Manneville; Alan Hall
Journal: Nature Date: 2002-12-12 Impact factor: 49.962

3. Fluorescence methods in the study of small GTP-binding proteins.

Authors: Mohammad Reza Ahmadian; Alfred Wittinghofer; Christian Herrmann
Journal: Methods Mol Biol Date: 2002

4. Crystal structures of a Formin Homology-2 domain reveal a tethered dimer architecture.

Authors: Yingwu Xu; James B Moseley; Isabelle Sagot; Florence Poy; David Pellman; Bruce L Goode; Michael J Eck
Journal: Cell Date: 2004-03-05 Impact factor: 41.582

5. The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization.

Authors: Atsushi Shimada; Miklós Nyitrai; Ingrid R Vetter; Dorothee Kühlmann; Beáta Bugyi; Shuh Narumiya; Michael A Geeves; Alfred Wittinghofer
Journal: Mol Cell Date: 2004-02-27 Impact factor: 17.970

6. p140mDia, a mammalian homolog of Drosophila diaphanous, is a target protein for Rho small GTPase and is a ligand for profilin.

Authors: N Watanabe; P Madaule; T Reid; T Ishizaki; G Watanabe; A Kakizuka; Y Saito; K Nakao; B M Jockusch; S Narumiya
Journal: EMBO J Date: 1997-06-02 Impact factor: 11.598

7. Solvent content of protein crystals.

Authors: B W Matthews
Journal: J Mol Biol Date: 1968-04-28 Impact factor: 5.469

Review 8. The guanine nucleotide-binding switch in three dimensions.

Authors: I R Vetter; A Wittinghofer
Journal: Science Date: 2001-11-09 Impact factor: 47.728

9. Kinetic and structural analysis of the Mg(2+)-binding site of the guanine nucleotide-binding protein p21H-ras.

Authors: J John; H Rensland; I Schlichting; I Vetter; G D Borasio; R S Goody; A Wittinghofer
Journal: J Biol Chem Date: 1993-01-15 Impact factor: 5.157

10. Characterization of functional domains of mDia1, a link between the small GTPase Rho and the actin cytoskeleton.

Authors: A Krebs; M Rothkegel; M Klar; B M Jockusch
Journal: J Cell Sci Date: 2001-10 Impact factor: 5.285

10 in total

6 in total

The purification and crystallization of mDia1 in complex with RhoC.

1. Identification of a carboxyl-terminal diaphanous-related formin homology protein autoregulatory domain.

Review 2. Rho GTPases in cell biology.

3. Fluorescence methods in the study of small GTP-binding proteins.

4. Crystal structures of a Formin Homology-2 domain reveal a tethered dimer architecture.

5. The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization.

6. p140mDia, a mammalian homolog of Drosophila diaphanous, is a target protein for Rho small GTPase and is a ligand for profilin.

7. Solvent content of protein crystals.

Review 8. The guanine nucleotide-binding switch in three dimensions.

9. Kinetic and structural analysis of the Mg(2+)-binding site of the guanine nucleotide-binding protein p21H-ras.

10. Characterization of functional domains of mDia1, a link between the small GTPase Rho and the actin cytoskeleton.

1. The regulation of mDia1 by autoinhibition and its release by Rho*GTP.

2. The citron homology domain as a scaffold for Rho1 signaling.

3. Specificity of interactions between mDia isoforms and Rho proteins.

4. Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec3.

5. The RhoGEF TEM4 Regulates Endothelial Cell Migration by Suppressing Actomyosin Contractility.

6. A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions.