Literature DB >> 16510996

Expression, purification, crystallization and preliminary crystallographic analysis of the PAB0955 gene product.

Stéphanie Gras1, Bernard Fernandez, Valérie Chaumont, Philippe Carpentier, Jean Armengaud, Dominique Housset.   

Abstract

PAB0955 from Pyrococcus abyssi is a prototype of a new Walker-type ATPase/GTPase conserved in archaea and eukaryota but not found in bacteria. PAB0955 has been expressed, purified and crystallized, and it has been shown that this thermostable protein is dimeric in reductive conditions. Crystals have been obtained either without nucleotide or in the presence of GDP or GTPgammaS. Preliminary X-ray crystallographic data up to 2.08 A resolution have been collected from these crystals.

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Year:  2005        PMID： 16510996      PMCID： PMC1952251          DOI： 10.1107/S1744309105000035

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  6 in total

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Authors:  O Matte-Tailliez; Y Zivanovic; P Forterre
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Journal:  J Biol Chem       Date:  2003-05-19       Impact factor: 5.157

3.  BEPITOPE: predicting the location of continuous epitopes and patterns in proteins.

Authors:  Michael Odorico; Jean-Luc Pellequer
Journal:  J Mol Recognit       Date:  2003 Jan-Feb       Impact factor: 2.137

4.  N2-methylation of guanosine at position 10 in tRNA is catalyzed by a THUMP domain-containing, S-adenosylmethionine-dependent methyltransferase, conserved in Archaea and Eukaryota.

Authors:  Jean Armengaud; Jaunius Urbonavicius; Bernard Fernandez; Guylaine Chaussinand; Janusz M Bujnicki; Henri Grosjean
Journal:  J Biol Chem       Date:  2004-06-20       Impact factor: 5.157

5.  The CCP4 suite: programs for protein crystallography.

Authors: 
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-09-01

6.  An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi.

Authors:  Georges N Cohen; Valérie Barbe; Didier Flament; Michael Galperin; Roland Heilig; Odile Lecompte; Olivier Poch; Daniel Prieur; Joël Quérellou; Raymond Ripp; Jean-Claude Thierry; John Van der Oost; Jean Weissenbach; Yvan Zivanovic; Patrick Forterre
Journal:  Mol Microbiol       Date:  2003-03       Impact factor: 3.501
  6 in total
  4 in total

1.  Structural insights into a new homodimeric self-activated GTPase family.

Authors:  Stéphanie Gras; Valérie Chaumont; Bernard Fernandez; Philippe Carpentier; Fabienne Charrier-Savournin; Sophie Schmitt; Charles Pineau; Didier Flament; Arnaud Hecker; Patrick Forterre; Jean Armengaud; Dominique Housset
Journal:  EMBO Rep       Date:  2007-04-20       Impact factor: 8.807

2.  Human GTPases associate with RNA polymerase II to mediate its nuclear import.

Authors:  Clément Carré; Ramin Shiekhattar
Journal:  Mol Cell Biol       Date:  2011-07-18       Impact factor: 4.272

3.  Eukaryotic GPN-loop GTPases paralogs use a dimeric assembly reminiscent of archeal GPN.

Authors:  Béatrice Alonso; Carole Beraud; Sarra Meguellati; Shu W Chen; Jean Luc Pellequer; Jean Armengaud; Christian Godon
Journal:  Cell Cycle       Date:  2013-01-16       Impact factor: 4.534

4.  Nuclear import of RNA polymerase II is coupled with nucleocytoplasmic shuttling of the RNA polymerase II-associated protein 2.

Authors:  Diane Forget; Andrée-Anne Lacombe; Philippe Cloutier; Mathieu Lavallée-Adam; Mathieu Blanchette; Benoit Coulombe
Journal:  Nucleic Acids Res       Date:  2013-05-30       Impact factor: 16.971
  4 in total

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