Literature DB >> 16510987

Expression, purification, crystallization and preliminary X-ray analysis of Aeromonas hydrophilia metallo-beta-lactamase.

Nandini Sharma1, Jeffrey H Toney, Paula M D Fitzgerald.   

Abstract

The CphA metallo-beta-lactamase from Aeromonas hydrophilia has been expressed, purified and crystallized by the hanging-drop vapor-diffusion method using ammonium sulfate as the precipitant. The crystals exhibit orthorhombic symmetry (P2(1)2(1)2), with unit-cell parameters a = 40.75, b = 42.05, c = 128.88 A. There is one monomer in the asymmetric unit and the solvent content is estimated to be 44% by volume. A data set extending to 1.8 A has been measured.

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Year:  2005        PMID： 16510987      PMCID： PMC1952252          DOI： 10.1107/S1744309104033512

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  7 in total

1.  Standard numbering scheme for class B beta-lactamases.

Authors:  M Galleni; J Lamotte-Brasseur; G M Rossolini; J Spencer; O Dideberg; J M Frère
Journal:  Antimicrob Agents Chemother       Date:  2001-03       Impact factor: 5.191

2.  Update of the standard numbering scheme for class B beta-lactamases.

Authors:  Gianpiero Garau; Isabel García-Sáez; Carine Bebrone; Christine Anne; Paola Mercuri; Moreno Galleni; Jean-Marie Frère; Otto Dideberg
Journal:  Antimicrob Agents Chemother       Date:  2004-07       Impact factor: 5.191

3.  Zn(II) dependence of the Aeromonas hydrophila AE036 metallo-beta-lactamase activity and stability.

Authors:  M Hernandez Valladares; A Felici; G Weber; H W Adolph; M Zeppezauer; G M Rossolini; G Amicosante; J M Frère; M Galleni
Journal:  Biochemistry       Date:  1997-09-23       Impact factor: 3.162

4.  Succinic acids as potent inhibitors of plasmid-borne IMP-1 metallo-beta-lactamase.

Authors:  J H Toney; G G Hammond; P M Fitzgerald; N Sharma; J M Balkovec; G P Rouen; S H Olson; M L Hammond; M L Greenlee; Y D Gao
Journal:  J Biol Chem       Date:  2001-06-04       Impact factor: 5.157

5.  Overproduction and purification of the Aeromonas hydrophila CphA metallo-beta-lactamase expressed in Escherichia coli.

Authors:  M Hernandez Villadares; M Galleni; J M Frère; A Felici; M Perilli; N Franceschini; G M Rossolini; A Oratore; G Amicosante
Journal:  Microb Drug Resist       Date:  1996       Impact factor: 3.431

6.  A metallo-beta-lactamase enzyme in action: crystal structures of the monozinc carbapenemase CphA and its complex with biapenem.

Authors:  Gianpiero Garau; Carine Bebrone; Christine Anne; Moreno Galleni; Jean-Marie Frère; Otto Dideberg
Journal:  J Mol Biol       Date:  2005-01-28       Impact factor: 5.469

7.  An overview of the kinetic parameters of class B beta-lactamases.

Authors:  A Felici; G Amicosante; A Oratore; R Strom; P Ledent; B Joris; L Fanuel; J M Frère
Journal:  Biochem J       Date:  1993-04-01       Impact factor: 3.857
  7 in total
  1 in total

1.  Mutagenesis of zinc ligand residue Cys221 reveals plasticity in the IMP-1 metallo-β-lactamase active site.

Authors:  Lori B Horton; Sreejesh Shanker; Rose Mikulski; Nicholas G Brown; Kevin J Phillips; Ernest Lykissa; B V Venkataram Prasad; Timothy Palzkill
Journal:  Antimicrob Agents Chemother       Date:  2012-08-20       Impact factor: 5.191
  1 in total

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