Literature DB >> 1651066

Chemiluminescent nucleic acid detection with digoxigenin-labeled probes: a model system with probes for angiotensin converting enzyme which detect less than one attomole of target DNA.

J J Lanzillo1.   

Abstract

Digoxigenin-labeled nucleic acid probes for angiotensin-converting enzyme were prepared by the polymerase chain reaction. In a model system, these probes were used to optimize conditions for target detection by direct chemiluminescence after dot-blot, Southern, or Northern hybridizations. Digoxigenin-labeled probes detected 23 fg (1.6 X 10(-19) mol or 9 X 10(4) copies) of target consistently.

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Year:  1991        PMID： 1651066     DOI： 10.1016/0003-2697(91)90149-n

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  10 in total

1.  Expression of renin and angiotensin-converting enzyme in human hearts.

Authors:  Y Endo-Mochizuki; N Mochizuki; H Sawa; A Takada; H Okamoto; H Kawaguchi; K Nagashima; A Kitabatake
Journal:  Heart Vessels       Date:  1995       Impact factor: 2.037

2.  A rapid and sensitive non-radioactive method applicable for genome-wide analysis of Saccharomyces cerevisiae genes involved in small RNA biology.

Authors:  Jingyan Wu; Hsiao-Yun Huang; Anita K Hopper
Journal:  Yeast       Date:  2013-03-12       Impact factor: 3.239

3.  Enhanced gene expression of transforming growth factor-alpha and c-met in rat urinary bladder cancer.

Authors:  M Inui; N Nishi; A Yasumoto; I Takenaka; H Miyanaka; K Matsumoto; T Nakamura; F Wada
Journal:  Urol Res       Date:  1996

4.  Genetic diversity of Desulfovibrio spp. in environmental samples analyzed by denaturing gradient gel electrophoresis of [NiFe] hydrogenase gene fragments.

Authors:  C Wawer; G Muyzer
Journal:  Appl Environ Microbiol       Date:  1995-06       Impact factor: 4.792

5.  Directly labeled DNA probes using fluorescent nucleotides with different length linkers.

Authors:  Z Zhu; J Chao; H Yu; A S Waggoner
Journal:  Nucleic Acids Res       Date:  1994-08-25       Impact factor: 16.971

6.  Gene duplication and multiplicity of collagenases in Clostridium histolyticum.

Authors:  O Matsushita; C M Jung; S Katayama; J Minami; Y Takahashi; A Okabe
Journal:  J Bacteriol       Date:  1999-02       Impact factor: 3.490

7.  3-Carboxy-cis,cis-muconate lactonizing enzyme from Neurospora crassa: an alternate cycloisomerase motif.

Authors:  P Mazur; W J Henzel; S Mattoo; J W Kozarich
Journal:  J Bacteriol       Date:  1994-03       Impact factor: 3.490

8.  Tumor necrosis factor alpha, interleukin 1 alpha, and interleukin 6 mRNA expressed by human astrocytoma cells after infection by three different strains of Toxoplasma gondii.

Authors:  H Pelloux; J Ricard; V Bracchi; Y Markowicz; J M Verna; P Ambroise-Thomas
Journal:  Parasitol Res       Date:  1994       Impact factor: 2.289

9.  Expression of Fas antigen and Fas ligand in bronchoalveolar lavage from silicosis patients.

Authors:  Agnès Hamzaoui; Jamel Ammar; Hédia Graïri; Kamel Hamzaoui
Journal:  Mediators Inflamm       Date:  2003-08       Impact factor: 4.711

10.  Isolation and characterization of invasive and noninvasive variants of a rat bladder tumor cell line.

Authors:  N Nishi; M Inui; Y Kishi; H Miyanaka; F Wada
Journal:  Jpn J Cancer Res       Date:  1997-09
  10 in total

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