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Literature DB >> 16508094

Crystallization and preliminary crystallographic analysis of endo-1,3-beta-glucanase from Arthrobacter sp.

Zhongcun Pang¹, You-Na Kang, Mizuho Ban, Masayuki Oda, Ryo Kobayashi, Masatake Ohnishi, Bunzo Mikami.

Abstract

Endo-1,3-beta-glucanases hydrolyze internal 1,3-beta-glucosyl linkages. The endo-1,3-beta-glucanase from Arthrobacter sp. was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group P4(1), with unit-cell parameters a = 71.31, c = 60.07 A, and contained one molecule per asymmetric unit. The Matthews coefficient (VM) and the solvent content were 2.35 A3 Da(-1) and 47.63%, respectively. Diffraction data were collected to a resolution of 1.66 A at SPring-8 using a MAR CCD area detector and gave a data set with an overall Rmerge of 5.4% and a completeness of 99.4%.

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Year: 2004 PMID： 16508094 PMCID： PMC1952366 DOI： 10.1107/S1744309104027915

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

17 in total

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5. Lic16A of Clostridium thermocellum, a non-cellulosomal, highly complex endo-beta-1,3-glucanase bound to the outer cell surface.

Authors: Klaus-Peter Fuchs; Vladimir V Zverlov; Galina A Velikodvorskaya; Friedrich Lottspeich; Wolfgang H Schwarz
Journal: Microbiology (Reading) Date: 2003-04 Impact factor: 2.777

Crystallization and preliminary crystallographic analysis of endo-1,3-beta-glucanase from Arthrobacter sp.

1. Crystal structure of a natural circularly permuted jellyroll protein: 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes.

Review 2. Immunomodulation and anti-cancer activity of polysaccharide-protein complexes.

3. Three N-terminal domains of beta-1,3-glucanase A1 are involved in binding to insoluble beta-1,3-glucan.

4. Processing of X-ray diffraction data collected in oscillation mode.

5. Lic16A of Clostridium thermocellum, a non-cellulosomal, highly complex endo-beta-1,3-glucanase bound to the outer cell surface.

6. Solvent content of protein crystals.

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8. Purification and characterization of laminaran hydrolases from Trichoderma viride.

9. Genes from Cellvibrio mixtus encoding beta-1,3 endoglucanase.

10. Crystal structure and site-directed mutagenesis of Bacillus macerans endo-1,3-1,4-beta-glucanase.

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