| Literature DB >> 1650783 |
H Collandre1, J T Aubin, H Agut, J M Béchet, L Montagnier.
Abstract
The polymerase chain reaction (PCR) was used to detect human herpes virus 6 (HHV-6) sequences in tissue culture. A pair of primers was synthesized and used to amplify a conserved region of the genome. Amplified products were detected either by visualization of UV illuminated ethidium bromide stained gel or, by hybridization with a specific radiolabeled oligonucleotide. As little as 5 fg of HHV-6 could be detected in infected cells, making this assay suitable for diagnostic purposes.Entities:
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Year: 1991 PMID： 1650783 DOI： 10.1016/0166-0934(91)90155-s
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014