OBJECTIVE: The underlying mechanisms of the changes in mental status, septic encephalopathy, and long-term cognitive symptoms in sepsis survivors have only been defined in part. The present study was undertaken to assess different variables of oxidative stress in several brain structures after cecal ligation and perforation in the rat. DESIGN: Prospective animal study. SETTING: Animal basic science laboratory. SUBJECTS: Male Wistar rats, weighing 250-350 g. INTERVENTIONS: Rats were subjected to cecal ligation and perforation (sepsis group) with saline resuscitation (at 50 mL/kg immediately and 12 hrs after cecal ligation and perforation) or sham operation (control group). MEASUREMENTS AND MAIN RESULTS: Oxidative damage, assessed by the thiobarbituric acid reactive species and the protein carbonyl assays, occurred early (after 6 hrs) in the course of sepsis development in the hippocampus, cerebellum, and cortex. At longer times after sepsis induction (12-96 hrs), there was no evidence of oxidative damage in all analyzed structures. Except for the striatum, earlier in sepsis development (6 hrs) we demonstrated an increase in superoxide dismutase activity without a proportional increase in catalase activity with a consequent increase in the relation of superoxide dismutase/catalase. The balance between these enzymes was restored in the studied structures 12-96 hrs after sepsis induction. CONCLUSIONS: The short-term oxidative damage demonstrated here could participate in the development of central nervous system symptoms during sepsis development, or even septic encephalopathy. The alterations in the superoxide dismutase/catalase relation were temporally related to the occurrence or not of oxidative damage in the central nervous system.
OBJECTIVE: The underlying mechanisms of the changes in mental status, septic encephalopathy, and long-term cognitive symptoms in sepsis survivors have only been defined in part. The present study was undertaken to assess different variables of oxidative stress in several brain structures after cecal ligation and perforation in the rat. DESIGN: Prospective animal study. SETTING: Animal basic science laboratory. SUBJECTS: Male Wistar rats, weighing 250-350 g. INTERVENTIONS:Rats were subjected to cecal ligation and perforation (sepsis group) with saline resuscitation (at 50 mL/kg immediately and 12 hrs after cecal ligation and perforation) or sham operation (control group). MEASUREMENTS AND MAIN RESULTS: Oxidative damage, assessed by the thiobarbituric acid reactive species and the protein carbonyl assays, occurred early (after 6 hrs) in the course of sepsis development in the hippocampus, cerebellum, and cortex. At longer times after sepsis induction (12-96 hrs), there was no evidence of oxidative damage in all analyzed structures. Except for the striatum, earlier in sepsis development (6 hrs) we demonstrated an increase in superoxide dismutase activity without a proportional increase in catalase activity with a consequent increase in the relation of superoxide dismutase/catalase. The balance between these enzymes was restored in the studied structures 12-96 hrs after sepsis induction. CONCLUSIONS: The short-term oxidative damage demonstrated here could participate in the development of central nervous system symptoms during sepsis development, or even septic encephalopathy. The alterations in the superoxide dismutase/catalase relation were temporally related to the occurrence or not of oxidative damage in the central nervous system.
Authors: Maira J da Cunha; Aline A da Cunha; Samanta O Loureiro; Fernanda R Machado; Felipe Schmitz; Janaína Kolling; Eduardo P Marques; Angela T S Wyse Journal: Mol Neurobiol Date: 2014-11-04 Impact factor: 5.590
Authors: Tatiana Barichello; Joelson C Lemos; Jaqueline S Generoso; Andreza L Cipriano; Graziele L Milioli; Danielle M Marcelino; Francieli Vuolo; Fabricia Petronilho; Felipe Dal-Pizzol; Márcia Carvalho Vilela; Antonio Lucio Teixeira Journal: Neurochem Res Date: 2011-06-03 Impact factor: 3.996
Authors: Mágada T Schwalm; Matheus Pasquali; Samantha P Miguel; João Paulo A Dos Santos; Francieli Vuolo; Clarissa M Comim; Fabrícia Petronilho; João Quevedo; Daniel P Gelain; José Cláudio F Moreira; Cristiane Ritter; Felipe Dal-Pizzol Journal: Mol Neurobiol Date: 2013-08-30 Impact factor: 5.590
Authors: Felipe Dal-Pizzol; Matheus Pasquali; João Quevedo; Daniel Pens Gelain; José Cláudio Fonseca Moreira Journal: Mol Med Date: 2012-12-20 Impact factor: 6.354
Authors: Francielle Mina; Clarissa M Comim; Diogo Dominguini; Omar J Cassol; Dhébora M Dall Igna; Gabriela K Ferreira; Milena C Silva; Leticia S Galant; Emílio L Streck; João Quevedo; Felipe Dal-Pizzol Journal: Mol Neurobiol Date: 2013-11-15 Impact factor: 5.590